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10.1074/jbc.271.40.24353 http://scihub22266oqcxt.onion/10.1074/jbc.271.40.24353 8798689!ä!8798689 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 1996 ; 271 (40): 24353-8 Nephropedia Template TP {{tp|p=8798689|t=1996. Angiotensin II and vasopressin modulate intracellular free magnesium in vascular smooth muscle cells through Na+-dependent protein kinase C pathways |pdf=|usr=}}{{8798689}} gab.com Text Angiotensin II and vasopressin modulate intracellular free magnesium in vascular smooth muscle cells through Na+-dependent protein kinase C pathways JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=8798689 #FOAvip Vasoactive peptides mobilize cytosolic free Mg2+ in vascular smooth muscle cells. It is unknown whether angiotensin II and arginine vasopressin, potent vasoconstrictor agents, influence intracellular Mg2+. The effects of angiotensin II and vasopressin on intracellular free Mg2+ concentrations ([Mg2+]i) were therefore investigated in primary cultured unpassaged vascular smooth muscle cells (VSMC) from mesenteric arteries of Wistar Kyoto rats, and in an established cell line of rat thoracic aorta cells (A10 cells). Underlying mechanisms of agonist-stimulated [Mg2+]i changes were assessed in A10 cells by pharmacologically manipulating phospholipase C, protein kinase C, and the Na+/H+ exchanger. In addition, the dependence of [Mg2+]i on intracellular Ca2+ was determined. [Mg2+]i was measured in single cells by fluorescent digital imaging using mag-fura-2/AM. Basal [Mg2+]i levels in Wistar Kyoto rat and A10 cells were 0.62 +/- 0.02 mmol/liter and 0.58 +/- 0.01 mmol/liter, respectively. Angiotensin II and vasopressin induced a dose-dependent biphasic [Mg2+]i response where [Mg2+]i increased rapidly and transiently to a peak level and then declined to subbasal levels, which were sustained. Preexposure of cells to neomycin, a nonspecific phospholipase C inhibitor, U-73122, a selective phospholipase C inhibitor, calphostin C, a selective protein kinase C inhibitor, and 5-(N, N-hexamethylene)amiloride, a selective Na+/H+ exchange blocker, attenuated angiotensin II- and vasopressin-induced [Mg2+]i responses in a concentration-dependent manner. Removal of extracellular Na+ completely inhibited agonist-elicited [Mg2+]i transients. To determine whether intracellular free Ca2+ concentration ([Ca2+]i) influences agonist-induced [Mg2+]i changes, thapsigargin, a selective sarcoplasmic reticular Ca2+-ATPase inhibitor, was used to deplete intracellular Ca2+ stores. In thapsigargin-pretreated cells, angiotensin II-elicited [Ca2+]i responses were significantly attenuated, whereas agonist-induced [Mg2+]i responses were unchanged. These data demonstrate that in primary cultured VSMC and in an established VSMC line, angiotensin II and vasopressin modulate [Mg2+]i through receptor-mediated pathways, which are [Ca2+]i-independent but which involve phospholipase C, protein kinase C, and the Na+/H+ exchanger. These pathways are linked to a Na+-dependent Mg2+ transporter, which facilitates transmembrane Mg2+ transport. Twit Text FOAVip Angiotensin II and vasopressin modulate intracellular free magnesium in vascular smooth muscle cells through Na+-dependent protein kinase C pathways ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=8798689 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=8798689 #FOAvip English Wikipedia <ref>{{Cite pmid|8798689}}</ref> Angiotensin II and vasopressin modulate intracellular free magnesium in vascular smooth muscle cells through Na+-dependent protein kinase C pathways #MMPMID8798689 Touyz RM; Schiffrin EL J Biol Chem 1996[Oct]; 271 (40): 24353-8 PMID8798689show ga Vasoactive peptides mobilize cytosolic free Mg2+ in vascular smooth muscle cells. It is unknown whether angiotensin II and arginine vasopressin, potent vasoconstrictor agents, influence intracellular Mg2+. The effects of angiotensin II and vasopressin on intracellular free Mg2+ concentrations ([Mg2+]i) were therefore investigated in primary cultured unpassaged vascular smooth muscle cells (VSMC) from mesenteric arteries of Wistar Kyoto rats, and in an established cell line of rat thoracic aorta cells (A10 cells). Underlying mechanisms of agonist-stimulated [Mg2+]i changes were assessed in A10 cells by pharmacologically manipulating phospholipase C, protein kinase C, and the Na+/H+ exchanger. In addition, the dependence of [Mg2+]i on intracellular Ca2+ was determined. [Mg2+]i was measured in single cells by fluorescent digital imaging using mag-fura-2/AM. Basal [Mg2+]i levels in Wistar Kyoto rat and A10 cells were 0.62 +/- 0.02 mmol/liter and 0.58 +/- 0.01 mmol/liter, respectively. Angiotensin II and vasopressin induced a dose-dependent biphasic [Mg2+]i response where [Mg2+]i increased rapidly and transiently to a peak level and then declined to subbasal levels, which were sustained. Preexposure of cells to neomycin, a nonspecific phospholipase C inhibitor, U-73122, a selective phospholipase C inhibitor, calphostin C, a selective protein kinase C inhibitor, and 5-(N, N-hexamethylene)amiloride, a selective Na+/H+ exchange blocker, attenuated angiotensin II- and vasopressin-induced [Mg2+]i responses in a concentration-dependent manner. Removal of extracellular Na+ completely inhibited agonist-elicited [Mg2+]i transients. To determine whether intracellular free Ca2+ concentration ([Ca2+]i) influences agonist-induced [Mg2+]i changes, thapsigargin, a selective sarcoplasmic reticular Ca2+-ATPase inhibitor, was used to deplete intracellular Ca2+ stores. In thapsigargin-pretreated cells, angiotensin II-elicited [Ca2+]i responses were significantly attenuated, whereas agonist-induced [Mg2+]i responses were unchanged. These data demonstrate that in primary cultured VSMC and in an established VSMC line, angiotensin II and vasopressin modulate [Mg2+]i through receptor-mediated pathways, which are [Ca2+]i-independent but which involve phospholipase C, protein kinase C, and the Na+/H+ exchanger. These pathways are linked to a Na+-dependent Mg2+ transporter, which facilitates transmembrane Mg2+ transport. |Angiotensin II/*pharmacology[MESH] |Animals[MESH] |Calcium/metabolism[MESH] |Cells, Cultured[MESH] |Magnesium/*metabolism[MESH] |Muscle, Smooth, Vascular/*drug effects/enzymology/metabolism[MESH] |Protein Kinase C/*metabolism[MESH] |Rats[MESH] |Rats, Inbred WKY[MESH] |Sodium/*metabolism[MESH]Angiotensin II and vasopressin modulate intracellular free magnesium i(epmc).pdf DeepDyve Pubget Overpricing