Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1007/BF01076575 http://scihub22266oqcxt.onion/10.1007/BF01076575 8569727!ä!8569727 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell+Biochem 1995 ; 149-150 (ä): 175-82 Nephropedia Template TP {{tp|p=8569727|t=1995. Second messenger role of magnesium in pancreatic acinar cells of the rat |pdf=|usr=}}{{8569727}} gab.com Text Second messenger role of magnesium in pancreatic acinar cells of the rat JO: Mol Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=8569727 #FOAvip Application of either acetylcholine (ACh, 10(-5) M) or cholecystokinin-octapeptide (CCK-8, 10(-8) M) to the isolated rat pancreas elicited large increases in amylase secretion, radiolabelled 45Ca2+ influx and cytosolic free calcium [Ca2+]i levels in zero and normal (1.1 mM) extracellular magnesium [Mg2+]o. Elevated [Mg2+]o significantly (p < 0.001) reduced the secretagogue-evoked secretory responses and Ca2+ mobilisation. Stimulation of pancreatic segments with either ACh (10(-5) and 10(-6) M) or CCK-8 (10(-8) and 10(-10) M) resulted in marked elevation in Mg2+ concentration in effluent samples (net efflux). On removal of either ACh or CCK-8, Mg2+ concentration returned to resting level. In pancreatic acinar cells loaded the fluorescent dye magfura, ACh and CCK-8 evoked marked reduction in cytosolic free Mg2+ concentration [Mg2+]i compared to the resting value of 0.82 +/- 0.03 mM (n = 50) in normal medium in the absence of secretagogues. In elevated [Mg2+]o (10 mM) medium, [Mg2+]i rises to 0.98 +/- 0.04 mM (n = 6). Addition of CCK-8 led to only a small reduction in [Mg2+]i in elevated [Mg2+]o. In Mg2+ loaded pancreatic acinar cells, Mg2+ is released in a time dependent manner and this efflux of Mg2+ was sensitive to sodium, extracellular amiloride (1 mM), dinitrophenol (10 mM) and lidocaine (1 mM). The results indicate that Mg2+ is acting as an intracellular messenger to regulate the mobilisation of Ca2+ which in turn mediates enzyme secretion. Twit Text FOAVip Second messenger role of magnesium in pancreatic acinar cells of the rat ????Mol Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=8569727 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=8569727 #FOAvip English Wikipedia <ref>{{Cite pmid|8569727}}</ref> Second messenger role of magnesium in pancreatic acinar cells of the rat #MMPMID8569727 Singh J; Wisdom DM Mol Cell Biochem 1995[Aug]; 149-150 (ä): 175-82 PMID8569727show ga Application of either acetylcholine (ACh, 10(-5) M) or cholecystokinin-octapeptide (CCK-8, 10(-8) M) to the isolated rat pancreas elicited large increases in amylase secretion, radiolabelled 45Ca2+ influx and cytosolic free calcium [Ca2+]i levels in zero and normal (1.1 mM) extracellular magnesium [Mg2+]o. Elevated [Mg2+]o significantly (p < 0.001) reduced the secretagogue-evoked secretory responses and Ca2+ mobilisation. Stimulation of pancreatic segments with either ACh (10(-5) and 10(-6) M) or CCK-8 (10(-8) and 10(-10) M) resulted in marked elevation in Mg2+ concentration in effluent samples (net efflux). On removal of either ACh or CCK-8, Mg2+ concentration returned to resting level. In pancreatic acinar cells loaded the fluorescent dye magfura, ACh and CCK-8 evoked marked reduction in cytosolic free Mg2+ concentration [Mg2+]i compared to the resting value of 0.82 +/- 0.03 mM (n = 50) in normal medium in the absence of secretagogues. In elevated [Mg2+]o (10 mM) medium, [Mg2+]i rises to 0.98 +/- 0.04 mM (n = 6). Addition of CCK-8 led to only a small reduction in [Mg2+]i in elevated [Mg2+]o. In Mg2+ loaded pancreatic acinar cells, Mg2+ is released in a time dependent manner and this efflux of Mg2+ was sensitive to sodium, extracellular amiloride (1 mM), dinitrophenol (10 mM) and lidocaine (1 mM). The results indicate that Mg2+ is acting as an intracellular messenger to regulate the mobilisation of Ca2+ which in turn mediates enzyme secretion. |Acetylcholine/physiology[MESH] |Amylases/metabolism[MESH] |Animals[MESH] |Biological Transport[MESH] |Calcium/metabolism[MESH] |Magnesium/*physiology[MESH] |Pancreas/*physiology[MESH] |Rats[MESH] |Second Messenger Systems[MESH] |Secretory Rate/drug effects[MESH] |Sincalide/pharmacology[MESH] |Sodium/physiology[MESH]Second messenger role of magnesium in pancreatic acinar cells of the r(epmc).pdf DeepDyve Pubget Overpricing