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http://scihub22266oqcxt.onion/10.1016/0014-5793(95)01439-x
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  • Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells derived from Japanese patients with chronic fatigue syndrome #MMPMID8549821
  • Nakaya T; Takahashi H; Nakamura Y; Asahi S; Tobiume M; Kuratsune H; Kitani T; Yamanishi K; Ikuta K
  • FEBS Lett 1996[Jan]; 378 (2): 145-9 PMID8549821garesp_yesshow ga
  • CFS, a recently named heterogeneous disorder, is an illness of unknown etiology. The association of CFS with viral infections has been suggested. A common association between CFS and several viruses examined has not been confirmed. Here, we centered on the possible link between CFS and BDV infection. By nested RT-PCR followed by hybridization, BDV RNA was demonstrated as a clear signal in PBMCs in 3 out of 25 CFS patients. The amplified cDNA fragments were cloned and sequenced. A total of 16 clones were studied. Intra-patients divergencies of the p24 were 2-9%, 3-20%, and 3-11% in the deduced amino acids. Inter-patient divergencies among the 16 clones were 3-24%. Antibodies to recombinant BDV p24 protein were detected in 6 CFS patients including one carrying BDV RNA. Overall, these gave the prevalence of 32% (8/25) in Japanese CFS patients, suggesting that Japanese CFS is highly associated with active infection of BDV, or a related agent.
  • |Adult[MESH]
  • |Amino Acid Sequence[MESH]
  • |Base Sequence[MESH]
  • |Blotting, Southern[MESH]
  • |Borna disease virus/genetics/*isolation & purification[MESH]
  • |DNA, Complementary/analysis[MESH]
  • |Fatigue Syndrome, Chronic/*virology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoblotting[MESH]
  • |Japan[MESH]
  • |Leukocytes, Mononuclear/*virology[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Molecular Sequence Data[MESH]
  • |Polymerase Chain Reaction[MESH]
  • |RNA, Viral/*blood[MESH]
  • |RNA-Directed DNA Polymerase[MESH]


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  • garesp_yesshow ga

    145 2.378 1996