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10.1016/0166-0934(93)90127-d http://scihub22266oqcxt.onion/10.1016/0166-0934(93)90127-d 8496295!?!8496295 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=8496295&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Virol+Methods 1993 ; 41 (2): 203-11 Nephropedia Template TP {{tp|p=8496295|t=1993. Efficient parvovirus B19 DNA purification and molecular cloning |pdf=|usr=}}{{8496295}} gab.com Text Efficient parvovirus B19 DNA purification and molecular cloning JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=8496295 #FOAvip A simple and efficient method of purification and molecular cloning of Parvovirus B19 DNA directly from small quantities of viremic sera was developed. Purified virions were lysed in annealing conditions, then viral DNA purification in double strand (ds) DNA form was achieved using an affinity DNA binding matrix. Affinity purification yielded a consistently high recovery of viral DNA. Using affinity purified ds viral DNA, we efficiently and stably cloned the complete coding internal unique sequence of B19 DNA. In our cloning strategy AatII and BamHI restriction endonuclease sites were exploited. This permitted cleavage of the 5.0 kbp AatII fragment in two AatII-BamHI fragments which could be efficiently cloned in a directional way in pUC18 plasmid vector. The availability of the two cloned AatII-BamHI fragments thus allowed the construction of a full length clone in a single ligation reaction. Twit Text FOAVip Efficient parvovirus B19 DNA purification and molecular cloning ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=8496295 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=8496295 #FOAvip English Wikipedia <ref>{{Cite pmid|8496295}}</ref> Efficient parvovirus B19 DNA purification and molecular cloning #MMPMID8496295 Gallinella G; Musiani M; Zerbini M; Gentilomi G; Gibellini D; Venturoli S; La Placa M J Virol Methods 1993[Feb]; 41 (2): 203-11 PMID8496295show ga A simple and efficient method of purification and molecular cloning of Parvovirus B19 DNA directly from small quantities of viremic sera was developed. Purified virions were lysed in annealing conditions, then viral DNA purification in double strand (ds) DNA form was achieved using an affinity DNA binding matrix. Affinity purification yielded a consistently high recovery of viral DNA. Using affinity purified ds viral DNA, we efficiently and stably cloned the complete coding internal unique sequence of B19 DNA. In our cloning strategy AatII and BamHI restriction endonuclease sites were exploited. This permitted cleavage of the 5.0 kbp AatII fragment in two AatII-BamHI fragments which could be efficiently cloned in a directional way in pUC18 plasmid vector. The availability of the two cloned AatII-BamHI fragments thus allowed the construction of a full length clone in a single ligation reaction. |Cloning, Molecular[MESH] |DNA, Viral/genetics/*isolation & purification[MESH] |Erythema Infectiosum/*microbiology[MESH] |Humans[MESH] |Parvovirus B19, Human/genetics/*isolation & purification[MESH]Efficient parvovirus B19 DNA purification and molecular cloning (epmc).pdf DeepDyve Pubget Overpricing