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10.1016/0165-2478(93)90114-h http://scihub22266oqcxt.onion/10.1016/0165-2478(93)90114-h 8300150!ä!8300150 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Immunol+Lett 1993 ; 38 (1): 26-31 Nephropedia Template TP {{tp|p=8300150|t=1993. Human recombinant GM-CSF selectively primes receptor mediated respiratory burst of neutrophils in vitro |pdf=|usr=}}{{8300150}} gab.com Text Human recombinant GM-CSF selectively primes receptor mediated respiratory burst of neutrophils in vitro JO: Immunol Lett http://www.kidney.de/pget.php?&tw=1&pmid=8300150 #FOAvip The influence of human recombinant granulocyte-macrophage colony-stimulating factor (rH GM-CSF) on respiratory burst response of isolated human neutrophils was examined. Preincubation of cells with rH GM-CSF significantly increased the respiratory burst in response to formyl-methionyl-leucyl-phenylalanine (FMLP), measured by luminol-dependent chemiluminescence (CL) assay. This priming effect of rH GM-CSF was independent of extracellular Ca2+ and Mg2+. On the other hand, the pretreatment of cells with rH GM-CSF could not enhance the neutrophil CL responses to unopsonized, serum complement-opsonized or immunoglobulin G (IgG)-opsonized zymosan particles. rH GM-CSF directly induced a weak CL signal in neutrophils. This signal, however, was abolished when extracellular Ca2+ and Mg2+ were removed. Exposure to rH GM-CSF caused a divalent cation-dependent up-regulation of complement receptors (CR1 and CR3) on neutrophil cell surface, while the expression of IgG Fc-receptors (FcRII and FcRIII) was not markedly changed by rH GM-CSF. The results indicate that rH GM-CSF primes FMLP-induced CL but not zymosan particle-induced respiratory burst in human neutrophils. It is hypothesized that the reason for the different sensitivity of FMLP-receptors and receptors to zymosan particles to rH GM-CSF priming may lie in differences in the signal-transduction pathways of these receptor types. Twit Text FOAVip Human recombinant GM-CSF selectively primes receptor mediated respiratory burst of neutrophils in vitro ????Immunol Lett http://www.kidney.de/pget.php?&tw=1&pmid=8300150 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=8300150 #FOAvip English Wikipedia <ref>{{Cite pmid|8300150}}</ref> Human recombinant GM-CSF selectively primes receptor mediated respiratory burst of neutrophils in vitro #MMPMID8300150 Leino L; Nuutila J; Pelliniemi TT; Rajamaki A Immunol Lett 1993[Sep]; 38 (1): 26-31 PMID8300150show ga The influence of human recombinant granulocyte-macrophage colony-stimulating factor (rH GM-CSF) on respiratory burst response of isolated human neutrophils was examined. Preincubation of cells with rH GM-CSF significantly increased the respiratory burst in response to formyl-methionyl-leucyl-phenylalanine (FMLP), measured by luminol-dependent chemiluminescence (CL) assay. This priming effect of rH GM-CSF was independent of extracellular Ca2+ and Mg2+. On the other hand, the pretreatment of cells with rH GM-CSF could not enhance the neutrophil CL responses to unopsonized, serum complement-opsonized or immunoglobulin G (IgG)-opsonized zymosan particles. rH GM-CSF directly induced a weak CL signal in neutrophils. This signal, however, was abolished when extracellular Ca2+ and Mg2+ were removed. Exposure to rH GM-CSF caused a divalent cation-dependent up-regulation of complement receptors (CR1 and CR3) on neutrophil cell surface, while the expression of IgG Fc-receptors (FcRII and FcRIII) was not markedly changed by rH GM-CSF. The results indicate that rH GM-CSF primes FMLP-induced CL but not zymosan particle-induced respiratory burst in human neutrophils. It is hypothesized that the reason for the different sensitivity of FMLP-receptors and receptors to zymosan particles to rH GM-CSF priming may lie in differences in the signal-transduction pathways of these receptor types. |Calcium/pharmacology[MESH] |Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology[MESH] |Humans[MESH] |Luminescent Measurements[MESH] |Magnesium/pharmacology[MESH] |N-Formylmethionine Leucyl-Phenylalanine/*pharmacology[MESH] |Neutrophils/*drug effects/physiology[MESH] |Opsonin Proteins/immunology[MESH] |Receptors, Formyl Peptide[MESH] |Receptors, Immunologic/drug effects/*physiology[MESH] |Receptors, Peptide/drug effects/*physiology[MESH] |Recombinant Proteins/pharmacology[MESH] |Respiratory Burst/*drug effects[MESH]Human recombinant GM-CSF selectively primes receptor mediated respirat(epmc).pdf DeepDyve Pubget Overpricing