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pmid8228234      J+Immunol 1993 ; 151 (10): 5416-24
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  • Effect of granulocyte/macrophage colony-stimulating factor on human monocytes infected with influenza A virus Enhancement of virus replication, cytokine release, and cytotoxicity #MMPMID8228234
  • Bender A; Amann U; Jager R; Nain M; Gemsa D
  • J Immunol 1993[Nov]; 151 (10): 5416-24 PMID8228234show ga
  • The activating properties of granulocyte/macrophage (GM)-CSF were studied in vitro with human monocytes infected by influenza A virus. When monocytes were pretreated for 8 h with GM-CSF (100 U/ml) and then exposed to influenza A virus, de novo virus protein synthesis was enhanced, more virus particles were released, and cells were killed at a higher rate. In virus-infected monocytes, GM-CSF induced a more rapid IFN-alpha release and potentiated production of TNF-alpha, IL-1 beta, and IL-6. Although GM-CSF or influenza A virus were each capable of independently activating TNF-alpha, IL-1 beta, and IL-6 gene transcription, a combination of both induced a massive cytokine mRNA accumulation which was readily translated into bioactive protein. Thus, GM-CSF may display a Janus-like action by accelerating virus infection but also by priming monocytes for elevated cytokine production. Whether the facilitated influenza A virus replication caused by GM-CSF may be counterbalanced by an improved cytokine response remains to be studied under more complex in vivo conditions.
  • |Base Sequence[MESH]
  • |Cells, Cultured[MESH]
  • |Cytokines/genetics/*metabolism[MESH]
  • |Cytotoxicity, Immunologic/*drug effects[MESH]
  • |Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology[MESH]
  • |Humans[MESH]
  • |Influenza A virus/*drug effects/physiology[MESH]
  • |Molecular Sequence Data[MESH]
  • |Monocytes/*drug effects/microbiology/physiology[MESH]
  • |Transcription, Genetic/drug effects[MESH]


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