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10.1099/0022-1317-75-6-1475

http://scihub22266oqcxt.onion/10.1099/0022-1317-75-6-1475
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8207413!ä!8207413

suck abstract from ncbi


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pmid8207413      J+Gen+Virol 1994 ; 75 ( Pt 6) (ä): 1475-8
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  • Differential transcription, without replication, of non-structural and structural genes of human parvovirus B19 in the UT7/EPO cell as demonstrated by in situ hybridization #MMPMID8207413
  • Leruez M; Pallier C; Vassias I; Elouet JF; Romeo P; Morinet F
  • J Gen Virol 1994[Jun]; 75 ( Pt 6) (ä): 1475-8 PMID8207413show ga
  • Erythroid progenitor cells are the main target for B19 parvovirus infection. The UT7 cell line demonstrates a marked erythroid differentiation on induction by erythropoietin (EPO) (UT7/EPO cells) and therefore appears to be a potential target for B19 parvovirus. We aimed to evaluate the presence and localization of B19 nucleic acids in UT7/EPO cells by in situ hybridization. Three digoxigenin-labelled probes were used: two recognized specifically the non-structural region of the B19 genome and one probe was structural region-specific. In our experiment UT7/EPO cells were not permissive to B19 infection. Transcription led to nonstructural and structural gene transcripts without DNA replication or capsid protein synthesis.
  • |*Gene Expression Regulation, Viral[MESH]
  • |Base Sequence[MESH]
  • |Cell Line[MESH]
  • |DNA Primers/chemistry[MESH]
  • |Genes, Viral[MESH]
  • |Humans[MESH]
  • |Molecular Sequence Data[MESH]
  • |Nucleic Acid Hybridization[MESH]
  • |Parvovirus B19, Human/*genetics[MESH]
  • |Transcription, Genetic[MESH]
  • |Viral Proteins/*genetics[MESH]
  • |Viral Structural Proteins/genetics[MESH]


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