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10.1099/0022-1317-76-5-1233 http://scihub22266oqcxt.onion/10.1099/0022-1317-76-5-1233 7730807!ä!7730807 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Gen+Virol 1995 ; 76 ( Pt 5) (ä): 1233-7 Nephropedia Template TP {{tp|p=7730807|t=1995. Morphology and antigenicity of recombinant B19 parvovirus capsids expressed in transfected COS-7 cells |pdf=|usr=}}{{7730807}} gab.com Text Morphology and antigenicity of recombinant B19 parvovirus capsids expressed in transfected COS-7 cells JO: J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=7730807 #FOAvip COS-7 cells transfected with parvovirus B19-simian virus 40 (SV40) hybrid vectors have previously been shown to express B19 structural proteins. In this study the morphology and antigenicity of B19 proteins expressed in these cells were investigated. At 84 h after transfection, approximately 10% of the COS-7 cells expressed B19 antigen, and the yield was equivalent to 2 x 10(3) to 2 x 10(5) B19 particles/transfected cell. The B19 proteins self-assembled into capsids that were morphologically and antigenically similar to native B19 virions, and could substitute for native antigen in a B19 IgM assay. Recombinant capsids lacking the recently described 11 kDa protein also resembled native virions. Twit Text FOAVip Morphology and antigenicity of recombinant B19 parvovirus capsids expressed in transfected COS-7 cells ????J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=7730807 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=7730807 #FOAvip English Wikipedia <ref>{{Cite pmid|7730807}}</ref> Morphology and antigenicity of recombinant B19 parvovirus capsids expressed in transfected COS-7 cells #MMPMID7730807 Cohen BJ; Field AM; Mori J; Brown KE; Clewley JP; St Amand J; Astell CR J Gen Virol 1995[May]; 76 ( Pt 5) (ä): 1233-7 PMID7730807show ga COS-7 cells transfected with parvovirus B19-simian virus 40 (SV40) hybrid vectors have previously been shown to express B19 structural proteins. In this study the morphology and antigenicity of B19 proteins expressed in these cells were investigated. At 84 h after transfection, approximately 10% of the COS-7 cells expressed B19 antigen, and the yield was equivalent to 2 x 10(3) to 2 x 10(5) B19 particles/transfected cell. The B19 proteins self-assembled into capsids that were morphologically and antigenically similar to native B19 virions, and could substitute for native antigen in a B19 IgM assay. Recombinant capsids lacking the recently described 11 kDa protein also resembled native virions. |Animals[MESH] |Antigens, Viral/immunology[MESH] |Capsid/*immunology/ultrastructure[MESH] |Cell Line[MESH] |Parvovirus B19, Human/*immunology[MESH] |Recombinant Proteins/immunology[MESH] |Simian virus 40/immunology[MESH]Morphology and antigenicity of recombinant B19 parvovirus capsids expr(epmc).pdf DeepDyve Pubget Overpricing