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10.1016/0166-6851(81)90030-x http://scihub22266oqcxt.onion/10.1016/0166-6851(81)90030-x 7322188!ä!7322188 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Biochem+Parasitol 1981 ; 4 (1-2): 67-76 Nephropedia Template TP {{tp|p=7322188|t=1981. Leishmania donovani-macrophage binding mediated by surface glycoproteins/antigens: characterization in vitro by a radioisotopic assay |pdf=|usr=}}{{7322188}} gab.com Text Leishmania donovani-macrophage binding mediated by surface glycoproteins/antigens: characterization in vitro by a radioisotopic assay JO: Mol Biochem Parasitol http://www.kidney.de/pget.php?&tw=1&pmid=7322188 #FOAvip A radioisotopic assay was developed to quantitate the binding of Leishmania donovani promastigotes to hamster peritoneal macrophages in vitro. The binding was temperature dependent and required no serum factors. Binding was reduced by preloading macrophages with zymosan granules or unlabeled promastigotes, but not with latex leads or opsonized erythrocytes. Binding was reduced by 10 mM EGTA that was reversible by the addition at an equimolar concentration of calcium, but not magnesium ions. Sialic acid, D-glucose, D-mannose and their derivatives reduced the binding, whereas L-fucose, D-galactose and their related sugars did not. Pretreatment of promastigotes with neuraminidase, alpha-mannosidase, alpha-N-acetylglucosaminidase or beta-glucosidase reduced their binding to macrophages. Prior trypsinization of either macrophages or promastigotes also substantially reduced the binding. At 4 degrees C, prior opsonization of promastigotes with subagglutination titers of antiserum doubled the level of binding but in combination with Protein A reduced it to 50% of its normal binding level. Prior opsonization of macrophages decreased their binding to promastigotes significantly at 4 or 37 degrees C. The results indicate that binding of Leishmania donovani promastigotes to hamster peritoneal macrophages is a ligand-receptor interaction involving their antigenic surface membrane proteins. The binding ligands of the parasites appear to have at least sialol, glucosyl, mannosyl and N-acetylglucosaminyl terminal residues as binding determinants. Thus, receptor-mediated endocytosis, defined in a broader sense, appears to be the mechanism by which leishmanias gain entry into macrophages. Twit Text FOAVip Leishmania donovani-macrophage binding mediated by surface glycoproteins/antigens: characterization in vitro by a radioisotopic assay ????Mol Biochem Parasitol http://www.kidney.de/pget.php?&tw=1&pmid=7322188 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=7322188 #FOAvip English Wikipedia <ref>{{Cite pmid|7322188}}</ref> Leishmania donovani-macrophage binding mediated by surface glycoproteins/antigens: characterization in vitro by a radioisotopic assay #MMPMID7322188 Chang KP Mol Biochem Parasitol 1981[Nov]; 4 (1-2): 67-76 PMID7322188show ga A radioisotopic assay was developed to quantitate the binding of Leishmania donovani promastigotes to hamster peritoneal macrophages in vitro. The binding was temperature dependent and required no serum factors. Binding was reduced by preloading macrophages with zymosan granules or unlabeled promastigotes, but not with latex leads or opsonized erythrocytes. Binding was reduced by 10 mM EGTA that was reversible by the addition at an equimolar concentration of calcium, but not magnesium ions. Sialic acid, D-glucose, D-mannose and their derivatives reduced the binding, whereas L-fucose, D-galactose and their related sugars did not. Pretreatment of promastigotes with neuraminidase, alpha-mannosidase, alpha-N-acetylglucosaminidase or beta-glucosidase reduced their binding to macrophages. Prior trypsinization of either macrophages or promastigotes also substantially reduced the binding. At 4 degrees C, prior opsonization of promastigotes with subagglutination titers of antiserum doubled the level of binding but in combination with Protein A reduced it to 50% of its normal binding level. Prior opsonization of macrophages decreased their binding to promastigotes significantly at 4 or 37 degrees C. The results indicate that binding of Leishmania donovani promastigotes to hamster peritoneal macrophages is a ligand-receptor interaction involving their antigenic surface membrane proteins. The binding ligands of the parasites appear to have at least sialol, glucosyl, mannosyl and N-acetylglucosaminyl terminal residues as binding determinants. Thus, receptor-mediated endocytosis, defined in a broader sense, appears to be the mechanism by which leishmanias gain entry into macrophages. |Animals[MESH] |Calcium[MESH] |Carbohydrates/pharmacology[MESH] |Cricetinae[MESH] |Glycoproteins/*metabolism[MESH] |Host-Parasite Interactions[MESH] |Hydrolases/pharmacology[MESH] |Kinetics[MESH] |Leishmania/immunology/*metabolism[MESH] |Macrophages/immunology/metabolism/*parasitology[MESH] |Membrane Proteins/*metabolism[MESH]Leishmania donovani-macrophage binding mediated by surface glycoprote(epmc).pdf DeepDyve Pubget Overpricing