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10.1021/acs.analchem.5c05037 http://scihub22266oqcxt.onion/10.1021/acs.analchem.5c05037 41359355!?!41359355 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=41359355&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Anal+Chem 2025 ; ? (?): ? Nephropedia Template TP {{tp|p=41359355|t=2025. Accurate Single-Particle Tracking and Diffusion Measurement in Freestanding Lipid Bilayers and Model Membranes |pdf=|usr=}}{{41359355}} gab.com Text Accurate Single-Particle Tracking and Diffusion Measurement in Freestanding Lipid Bilayers and Model Membranes JO: Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=41359355 #FOAvip Single-particle tracking (SPT) in lipid bilayers offers a powerful means to investigate the dynamic interaction of individual molecules in a variety of model membranes. However, accurate diffusion characterization in freestanding bilayers remains challenging due to membrane fragility, the potential for curvature, limited optical signal-to-background ratio, and the practical need for label concentrations that do not approximate infinite dilution. Here, we report a dual-mode fluorescence microscope configuration for bilayers formed on a microelectrode cavity array (MECAopto-inv chip) that combines widefield SPT with confocal fluorescence correlation spectroscopy (FCS). By using FCS to calibrate the SPT algorithm, we demonstrate improved measurement accuracy for diffusion constants. Importantly, we show that proper calibration resists measurement bias at high fluorescent particle concentrations. Supporting simulations confirm the robustness of this approach. When calibrated with FCS and corrected for blur, our measurements help resolve the variability in diffusion constants reported in prior literature. Furthermore, the strategy is broadly applicable to any planar bilayer system. The method provides a rigorous, quantitative benchmark for future studies involving nanopores, membrane protein assemblies, and ion-channel transport in lipid bilayers, especially when both optical and electrical information is desired. Twit Text FOAVip Accurate Single-Particle Tracking and Diffusion Measurement in Freestanding Lipid Bilayers and Model Membranes ????Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=41359355 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=41359355 #FOAvip English Wikipedia <ref>{{Cite pmid|41359355}}</ref> Accurate Single-Particle Tracking and Diffusion Measurement in Freestanding Lipid Bilayers and Model Membranes #MMPMID41359355 Van Ye LA; Michael RD; Meyer JJ; Pena SM; Bailey DJ; Keranen-Burden LM; Burden DL Anal Chem 2025[Dec]; ? (?): ? PMID41359355show ga Single-particle tracking (SPT) in lipid bilayers offers a powerful means to investigate the dynamic interaction of individual molecules in a variety of model membranes. However, accurate diffusion characterization in freestanding bilayers remains challenging due to membrane fragility, the potential for curvature, limited optical signal-to-background ratio, and the practical need for label concentrations that do not approximate infinite dilution. Here, we report a dual-mode fluorescence microscope configuration for bilayers formed on a microelectrode cavity array (MECAopto-inv chip) that combines widefield SPT with confocal fluorescence correlation spectroscopy (FCS). By using FCS to calibrate the SPT algorithm, we demonstrate improved measurement accuracy for diffusion constants. Importantly, we show that proper calibration resists measurement bias at high fluorescent particle concentrations. Supporting simulations confirm the robustness of this approach. When calibrated with FCS and corrected for blur, our measurements help resolve the variability in diffusion constants reported in prior literature. Furthermore, the strategy is broadly applicable to any planar bilayer system. The method provides a rigorous, quantitative benchmark for future studies involving nanopores, membrane protein assemblies, and ion-channel transport in lipid bilayers, especially when both optical and electrical information is desired. ?Accurate Single-Particle Tracking and Diffusion Measurement in Freesta(epmc).pdf DeepDyve Pubget Overpricing