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ECT10 is an m(6)A reader that negatively regulates the drought stress response in Arabidopsis by modulating mRNA stability #MMPMID41352334
Han R; Nguyen TKH; Yang Z; Kang H
Plant J 2025[Dec]; 124 (5): e70637 PMID41352334show ga
N(6)-methyladenosine (m(6)A) modification occurs prevalently in eukaryotic mRNAs and is a key epitranscriptomic mechanism regulating plant development and stress responses. The m(6)A mark is interpreted by 'readers' that recognize and bind to m(6)A-modified RNAs. Recent studies highlight Evolutionarily Conserved C-terminal region (ECT) proteins as m(6)A readers in various plant species. However, the functions of many ECT proteins remain unknown. Therefore, this study aims to determine the role of ECT10 as an m(6)A reader and to evaluate its function in drought stress response in Arabidopsis. The electrophoretic mobility shift assay demonstrated that ECT10 specifically binds to m(6)A-modified RNAs but not to m(5)C-modified RNAs in vitro. The expression of ECT10 decreased markedly under drought stress conditions. The ect10 loss-of-function mutant exhibited reduced sensitivity to drought stress. Complementation lines expressing the native ECT10 in the ect10 mutant background restored the wild-type phenotype, while lines expressing the mutant mECT10, harboring substitutions at two critical amino acid residues, failed to rescue the phenotype. ECT10 regulated the expression of several m(6)A-modified positive or negative regulators of the drought stress response by modulating their stability through direct binding to these m(6)A-modified transcripts in planta. Collectively, these findings establish ECT10 as a novel m(6)A reader crucial for drought stress response by modulating mRNA stability.
Plant+J 2025 ; 124 (5): e70637
