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10.1002/smtd.202501936 http://scihub22266oqcxt.onion/10.1002/smtd.202501936 41351211!?!41351211 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=41351211&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Small+Methods 2025 ; ? (?): e01936 Nephropedia Template TP {{tp|p=41351211|t=2025. Characterization of Non-Specific Electrostatic Interactions of Cationic Peptides with DNA Origami and Their Functional Consequences |pdf=|usr=}}{{41351211}} gab.com Text Characterization of Non-Specific Electrostatic Interactions of Cationic Peptides with DNA Origami and Their Functional Consequences JO: Small Methods http://www.kidney.de/pget.php?&tw=1&pmid=41351211 #FOAvip The functionalization of DNA origami with peptides is a powerful strategy for creating nanodevices for therapeutic and diagnostic applications. A critical but often overlooked challenge is the non-specific electrostatic binding of cationic peptides to the anionic DNA nanostructure, which leads to uncontrolled stoichiometry and undermines functional predictability. Here, the study systematically characterizes this issue and demonstrates a practical purification strategy to mitigate it. It is quantitatively shown that cationic peptides associate with DNA origami in vast excess of their intended binding sites, a phenomenon not observed with anionic control peptides. This non-specific binding is confirmed to be electrostatic and is effectively screened by high salt. To address this, a charge-dependent purification approach is evaluated using polyethylene glycol (PEG) precipitation, showing that cationic peptides require extensive purification (>/=7 cycles), whereas anionic peptides need only minimal treatment (2 cycles) to achieve precise loading. Crucially, the study provides definitive functional evidence that a therapeutic peptide (brain-derived neurotrophic factor-mimicking peptide) must be attached via stable, site-specific hybridization to elicit a potent biological response; non-specifically adsorbed peptides are largely inactive. This work provides a set of critical design guidelines and purification considerations necessary for the rational design of reliable and functionally predictable DNA nanodevices. Twit Text FOAVip Characterization of Non-Specific Electrostatic Interactions of Cationic Peptides with DNA Origami and Their Functional Consequences ????Small Methods http://www.kidney.de/pget.php?&tw=1&pmid=41351211 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=41351211 #FOAvip English Wikipedia <ref>{{Cite pmid|41351211}}</ref> Characterization of Non-Specific Electrostatic Interactions of Cationic Peptides with DNA Origami and Their Functional Consequences #MMPMID41351211 Kang SH; Kwon O; Cho BK; Yoo S; Wang JM; Choi Y; Yoon HY; Choi J; Ryu JH Small Methods 2025[Dec]; ? (?): e01936 PMID41351211show ga The functionalization of DNA origami with peptides is a powerful strategy for creating nanodevices for therapeutic and diagnostic applications. A critical but often overlooked challenge is the non-specific electrostatic binding of cationic peptides to the anionic DNA nanostructure, which leads to uncontrolled stoichiometry and undermines functional predictability. Here, the study systematically characterizes this issue and demonstrates a practical purification strategy to mitigate it. It is quantitatively shown that cationic peptides associate with DNA origami in vast excess of their intended binding sites, a phenomenon not observed with anionic control peptides. This non-specific binding is confirmed to be electrostatic and is effectively screened by high salt. To address this, a charge-dependent purification approach is evaluated using polyethylene glycol (PEG) precipitation, showing that cationic peptides require extensive purification (>/=7 cycles), whereas anionic peptides need only minimal treatment (2 cycles) to achieve precise loading. Crucially, the study provides definitive functional evidence that a therapeutic peptide (brain-derived neurotrophic factor-mimicking peptide) must be attached via stable, site-specific hybridization to elicit a potent biological response; non-specifically adsorbed peptides are largely inactive. This work provides a set of critical design guidelines and purification considerations necessary for the rational design of reliable and functionally predictable DNA nanodevices. ?Characterization of Non-Specific Electrostatic Interactions of Cationi(epmc).pdf DeepDyve Pubget Overpricing