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10.1016/j.ijbiomac.2025.146696 http://scihub22266oqcxt.onion/10.1016/j.ijbiomac.2025.146696 40784401!ä!40784401 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 334.4 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\40784401.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Int+J+Biol+Macromol 2025 ; 322 (Pt 2): 146696 Nephropedia Template TP {{tp|p=40784401|t=2025. Mining the whole genome sequence of Streptomyces cyaneofuscatus strain CTM50504 isolated from the Ain El-Atrous hot spring, Tunisia, for the discovery of extremozymes: Promising properties of protease activity |pdf=|usr=}}{{40784401}} gab.com Text Mining the whole genome sequence of Streptomyces cyaneofuscatus strain CTM50504 isolated from the Ain El-Atrous hot spring, Tunisia, for the discovery of extremozymes: Promising properties of protease activity JO: Int J Biol Macromol http://www.kidney.de/pget.php?&tw=1&pmid=40784401 #FOAvip Next-generation sequencing (NGS) methods allow for the generation of data leading to a greater understanding of the potential functionality and dynamics of microorganisms within their biotopes. The enormous volume of data that NGS produces necessitates understanding structural and functional genomics through the application of various omics techniques. Streptomyces cyaneofuscatus CTM50504 is a potential extracellular hydrolase producer isolated from a terrestrial hot spring, Ain El-Atrous, Korbous (Nabeul, Tunisia). This strain can grow at 50 degrees C and a pH range of 6-9. It requires the presence of NaCl for growth and secretes proteases, lipases, phospholipases, amylases, and chitinases. Whole-genome sequence (WGS) analysis was performed on strain CTM50504 to identify protein-encoding genes, including hydrolases. The genome sequence was assembled into 858-contigs with an average G + C content of 71 % and a total length of 8,591,922-bp. Genome annotation revealed 770-protein-coding genes with 323 open reading frames encoding hydrolases, including 179-proteases, 20-lipases, 10-phospholipases, 5-amylases, and 5-chitinases. The gene encoding a serine alkaline protease (SCKP) was heterologously expressed in Escherichia coli. The recombinant SCKP (rSCKP) was purified by affinity chromatography, and its biochemical properties were determined. Molecular dynamics simulations provided deeper insights into how key amino acids contribute to substrate binding and elucidated the basis of substrate selectivity. Twit Text FOAVip Mining the whole genome sequence of Streptomyces cyaneofuscatus strain CTM50504 isolated from the Ain El-Atrous hot spring, Tunisia, for the discovery of extremozymes: Promising properties of protease activity ????Int J Biol Macromol http://www.kidney.de/pget.php?&tw=1&pmid=40784401 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=40784401 #FOAvip English Wikipedia <ref>{{Cite pmid|40784401}}</ref> Mining the whole genome sequence of Streptomyces cyaneofuscatus strain CTM50504 isolated from the Ain El-Atrous hot spring, Tunisia, for the discovery of extremozymes: Promising properties of protease activity #MMPMID40784401 Mechri S; Croze S; Rekik I; Allala F; Frikha F; Noiriel A; Le Roes-Hill M; Abousalham A; Tounsi S; Lachuer J; Jaouadi B Int J Biol Macromol 2025[Aug]; 322 (Pt 2): 146696 PMID40784401show ga Next-generation sequencing (NGS) methods allow for the generation of data leading to a greater understanding of the potential functionality and dynamics of microorganisms within their biotopes. The enormous volume of data that NGS produces necessitates understanding structural and functional genomics through the application of various omics techniques. Streptomyces cyaneofuscatus CTM50504 is a potential extracellular hydrolase producer isolated from a terrestrial hot spring, Ain El-Atrous, Korbous (Nabeul, Tunisia). This strain can grow at 50 degrees C and a pH range of 6-9. It requires the presence of NaCl for growth and secretes proteases, lipases, phospholipases, amylases, and chitinases. Whole-genome sequence (WGS) analysis was performed on strain CTM50504 to identify protein-encoding genes, including hydrolases. The genome sequence was assembled into 858-contigs with an average G + C content of 71 % and a total length of 8,591,922-bp. Genome annotation revealed 770-protein-coding genes with 323 open reading frames encoding hydrolases, including 179-proteases, 20-lipases, 10-phospholipases, 5-amylases, and 5-chitinases. The gene encoding a serine alkaline protease (SCKP) was heterologously expressed in Escherichia coli. The recombinant SCKP (rSCKP) was purified by affinity chromatography, and its biochemical properties were determined. Molecular dynamics simulations provided deeper insights into how key amino acids contribute to substrate binding and elucidated the basis of substrate selectivity. äMining the whole genome sequence of Streptomyces cyaneofuscatus strain(epmc).pdf DeepDyve Pubget Overpricing