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10.1186/s12958-025-01428-6 http://scihub22266oqcxt.onion/10.1186/s12958-025-01428-6 40611176!ä!40611176 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\40611176.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Reprod+Biol+Endocrinol 2025 ; 23 (1): 93 Nephropedia Template TP {{tp|p=40611176|t=2025. Rapamycin reduces DNA damage of in vitro matured oocytes by promoting Rad51-mediated homologous recombination |pdf=|usr=}}{{40611176}} gab.com Text Rapamycin reduces DNA damage of in vitro matured oocytes by promoting Rad51-mediated homologous recombination JO: Reprod Biol Endocrinol http://www.kidney.de/pget.php?&tw=1&pmid=40611176 #FOAvip BACKGROUND: The quality of in vitro matured (IVM) oocytes remains inferior to that of in vivo matured oocytes, likely due to DNA damage induced by the in vitro environment. Although rapamycin has been shown to improve the developmental competence of IVM oocytes by reducing DNA double-strand breaks (DSBs), the underlying mechanisms remain unclear. METHODS: Immature oocytes from 6 ~ 8-week-old ICR female mice were subjected to IVM with or without rapamycin. After 14 ~ 16 h, the maturation rate, DNA DSB levels, and subsequent developmental competence of IVM oocytes were assessed. The homologous recombination (HR) and non-homologous end joining (NHEJ) pathways were selectively inhibited using RAD51 and DNA-PK inhibitors, respectively, to elucidate the predominant DNA damage repair pathway during IVM and investigate the effects of rapamycin on this process. RESULTS: Embryos derived from the rapamycin-treated group exhibited significantly higher 2PN and cleavage rates compared to the control group. MII oocytes cultured with rapamycin showed reduced gammaH2AX immunofluorescence intensity, indicating lower DNA damage levels. Additionally, the expression levels of RAD51 and DNAPK were elevated in rapamycin-treated oocytes. Inhibition of RAD51 significantly reduced the maturation rate and increased DNA damage levels, whereas DNAPK inhibition had no effect on oocyte development or quality. Importantly, the beneficial effects of rapamycin on IVM oocytes were diminished under RAD51 inhibition. CONCLUSION: Rapamycin improves the developmental competence of IVM oocytes by enhancing the RAD51-mediated HR pathway, thereby enhancing DNA stability. Twit Text FOAVip Rapamycin reduces DNA damage of in vitro matured oocytes by promoting Rad51-mediated homologous recombination ????Reprod Biol Endocrinol http://www.kidney.de/pget.php?&tw=1&pmid=40611176 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=40611176 #FOAvip English Wikipedia <ref>{{Cite pmid|40611176}}</ref> Rapamycin reduces DNA damage of in vitro matured oocytes by promoting Rad51-mediated homologous recombination #MMPMID40611176 Yang Q; Chen Y; Huang J; Tang J; Zhu L; Li J; Tong C Reprod Biol Endocrinol 2025[Jul]; 23 (1): 93 PMID40611176show ga BACKGROUND: The quality of in vitro matured (IVM) oocytes remains inferior to that of in vivo matured oocytes, likely due to DNA damage induced by the in vitro environment. Although rapamycin has been shown to improve the developmental competence of IVM oocytes by reducing DNA double-strand breaks (DSBs), the underlying mechanisms remain unclear. METHODS: Immature oocytes from 6 ~ 8-week-old ICR female mice were subjected to IVM with or without rapamycin. After 14 ~ 16 h, the maturation rate, DNA DSB levels, and subsequent developmental competence of IVM oocytes were assessed. The homologous recombination (HR) and non-homologous end joining (NHEJ) pathways were selectively inhibited using RAD51 and DNA-PK inhibitors, respectively, to elucidate the predominant DNA damage repair pathway during IVM and investigate the effects of rapamycin on this process. RESULTS: Embryos derived from the rapamycin-treated group exhibited significantly higher 2PN and cleavage rates compared to the control group. MII oocytes cultured with rapamycin showed reduced gammaH2AX immunofluorescence intensity, indicating lower DNA damage levels. Additionally, the expression levels of RAD51 and DNAPK were elevated in rapamycin-treated oocytes. Inhibition of RAD51 significantly reduced the maturation rate and increased DNA damage levels, whereas DNAPK inhibition had no effect on oocyte development or quality. Importantly, the beneficial effects of rapamycin on IVM oocytes were diminished under RAD51 inhibition. CONCLUSION: Rapamycin improves the developmental competence of IVM oocytes by enhancing the RAD51-mediated HR pathway, thereby enhancing DNA stability. |*DNA Damage/drug effects[MESH] |*Homologous Recombination/drug effects[MESH] |*In Vitro Oocyte Maturation Techniques/methods[MESH] |*Oocytes/drug effects/metabolism[MESH] |*Rad51 Recombinase/metabolism[MESH] |*Sirolimus/pharmacology[MESH] |Animals[MESH] |DNA Breaks, Double-Stranded/drug effects[MESH] |Female[MESH] |Mice[MESH]Rapamycin reduces DNA damage of in vitro matured oocytes by promoting (epmc).pdf DeepDyve Pubget Overpricing