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10.1016/0042-6822(87)90297-2 http://scihub22266oqcxt.onion/10.1016/0042-6822(87)90297-2 3824910!ä!3824910 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 1987 ; 157 (2): 539-42 Nephropedia Template TP {{tp|p=3824910|t=1987. A transcriptional promoter of the human parvovirus B19 active in vitro and in vivo |pdf=|usr=}}{{3824910}} gab.com Text A transcriptional promoter of the human parvovirus B19 active in vitro and in vivo JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=3824910 #FOAvip The human parvovirus B19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. So far, it has not been possible to grow B19 virus in cultured cells. Here we report the use of in vitro transcription in HeLa cell extracts and transient expression of cloned DNA transfected into HeLa cells to detect and map a strong transcriptional promoter on the B19 genome. The promoter is located near the left end of the B19 genome, at position 6 map units in the clone pYT103 (approximately 280 bp upstream of the first HindIII site), and directs transcription to the right. These results suggest that the strictly limited host range of B19 does not operate at the level of transcription from the promoter at the left end of the genome. Twit Text FOAVip A transcriptional promoter of the human parvovirus B19 active in vitro and in vivo ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=3824910 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=3824910 #FOAvip English Wikipedia <ref>{{Cite pmid|3824910}}</ref> A transcriptional promoter of the human parvovirus B19 active in vitro and in vivo #MMPMID3824910 Doerig C; Beard P; Hirt B Virology 1987[Apr]; 157 (2): 539-42 PMID3824910show ga The human parvovirus B19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. So far, it has not been possible to grow B19 virus in cultured cells. Here we report the use of in vitro transcription in HeLa cell extracts and transient expression of cloned DNA transfected into HeLa cells to detect and map a strong transcriptional promoter on the B19 genome. The promoter is located near the left end of the B19 genome, at position 6 map units in the clone pYT103 (approximately 280 bp upstream of the first HindIII site), and directs transcription to the right. These results suggest that the strictly limited host range of B19 does not operate at the level of transcription from the promoter at the left end of the genome. |*Promoter Regions, Genetic[MESH] |*Transcription, Genetic[MESH] |DNA, Viral/genetics[MESH] |Genes, Viral[MESH] |HeLa Cells[MESH] |Humans[MESH] |Parvoviridae/*genetics[MESH]A transcriptional promoter of the human parvovirus B19 active in vitro(epmc).pdf DeepDyve Pubget Overpricing