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10.1126/science.3738514

http://scihub22266oqcxt.onion/10.1126/science.3738514
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3738514!?!3738514

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suck abstract from ncbi

pmid3738514      Science 1986 ; 233 (4766): 883-6
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  • Replication of the B19 parvovirus in human bone marrow cell cultures #MMPMID3738514
  • Ozawa K; Kurtzman G; Young N
  • Science 1986[Aug]; 233 (4766): 883-6 PMID3738514show ga
  • The B19 parvovirus is responsible for at least three human diseases. The virus was successfully propagated in suspension cultures of human erythroid bone marrow from patients with hemolytic anemias; release of newly synthesized virus into the supernatants of infected cultures was observed. This culture system allowed study at a molecular level of events associated with the B19 life cycle. The B19 parvovirus replicated through high molecular weight intermediate forms, linked through a terminal hairpin structure. B19 replication in vitro was highly dependent on the erythropoietic content of cultures and on addition of the hormone erythropoietin.
  • |Anemia, Hemolytic/*microbiology[MESH]
  • |Bone Marrow/*microbiology[MESH]
  • |Cells, Cultured[MESH]
  • |Culture Media[MESH]
  • |DNA, Viral/analysis[MESH]
  • |Erythropoietin/metabolism[MESH]
  • |Humans[MESH]
  • |Parvoviridae/*growth & development[MESH]


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