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10.1016/j.isci.2022.105514 http://scihub22266oqcxt.onion/10.1016/j.isci.2022.105514 36425766!9678738!36425766     free     free     free       iScience 2022 ; 25 (12): 105514 Nephropedia Template TP {{tp|p=36425766|t=2022. Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages |pdf=|usr=}}{{36425766}} gab.com Text Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages JO: iScience http://www.kidney.de/pget.php?&tw=1&pmid=36425766 #FOAvip Podosomes, an important actin-based adhesive architecture, play critical roles in cell migration and matrix invasiveness. Here, we elucidate the ultrastructural organization and regulation of podosome clusters in primary macrophages. With three-dimensional stochastic optical reconstruction microscopy (3D-STORM), we achieve approximately 20/50 nm (lateral/axial) spatial resolution to resolve the mutual localization of podosome core and ring components, and further show that microtubules pass through podosomes at the layer of myosin IIA. The microtubule disruption-caused podosome dissolution is previously ascribed to Rho/ROCK-myosin signaling, yet inhibiting this pathway with Y27632 or blebbistatin only partially recovers podosome assembly, thus suggesting the contribution of the physical supporting of microtubules in stabilizing podosome structures. Through improved substrate-coating technique, we further corroborate that the matrix-degrading capability of macrophages depends on the formation of podosome clusters. Together, 3D-STORM super-resolution microscopy reveals the nanoscale spatial arrangement and the microtubule-dependent regulation of the matrix-degrading podosome clusters in macrophages. Twit Text FOAVip Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages ????iScience http://www.kidney.de/pget.php?&tw=1&pmid=36425766 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=36425766 #FOAvip English Wikipedia <ref>{{Cite pmid|36425766}}</ref> Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages #MMPMID36425766 Hu F; Zhu D; Dong H; Zhang P; Xing F; Li W; Yan R; Zhou J; Xu K; Pan L; Xu J iScience 2022[Dec]; 25 (12): 105514 PMID36425766show ga Podosomes, an important actin-based adhesive architecture, play critical roles in cell migration and matrix invasiveness. Here, we elucidate the ultrastructural organization and regulation of podosome clusters in primary macrophages. With three-dimensional stochastic optical reconstruction microscopy (3D-STORM), we achieve approximately 20/50 nm (lateral/axial) spatial resolution to resolve the mutual localization of podosome core and ring components, and further show that microtubules pass through podosomes at the layer of myosin IIA. The microtubule disruption-caused podosome dissolution is previously ascribed to Rho/ROCK-myosin signaling, yet inhibiting this pathway with Y27632 or blebbistatin only partially recovers podosome assembly, thus suggesting the contribution of the physical supporting of microtubules in stabilizing podosome structures. Through improved substrate-coating technique, we further corroborate that the matrix-degrading capability of macrophages depends on the formation of podosome clusters. Together, 3D-STORM super-resolution microscopy reveals the nanoscale spatial arrangement and the microtubule-dependent regulation of the matrix-degrading podosome clusters in macrophages. ?Super-resolution microscopy reveals nanoscale architecture and regulat(epmc).pdf DeepDyve Pubget Overpricing