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10.7554/eLife.83346 http://scihub22266oqcxt.onion/10.7554/eLife.83346 36382883!9691018!36382883     free     free     free       Elife 2022 ; 11 (?): ? Nephropedia Template TP {{tp|p=36382883|t=2022. Reevaluation of Piezo1 as a gut RNA sensor |pdf=|usr=}}{{36382883}} gab.com Text Reevaluation of Piezo1 as a gut RNA sensor JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=36382883 #FOAvip Piezo1 is a stretch-gated ion channel required for mechanosensation in many organ systems. Recent findings point to a new role for Piezo1 in the gut, suggesting that it is a sensor of microbial single-stranded RNA (ssRNA) rather than mechanical force. If true, this would redefine the scope of Piezo biology. Here, we sought to replicate the central finding that fecal ssRNA is a natural agonist of Piezo1. While we observe that fecal extracts and ssRNA can stimulate calcium influx in certain cell lines, this response is independent of Piezo1. Additionally, sterilized dietary extracts devoid of gut biome RNA show similar cell line-specific stimulatory activity to fecal extracts. Together, our data highlight potential confounds inherent to gut-derived extracts, exclude Piezo1 as a receptor for ssRNA in the gut, and support a dedicated role for Piezo channels in mechanosensing. Twit Text FOAVip Reevaluation of Piezo1 as a gut RNA sensor ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=36382883 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=36382883 #FOAvip English Wikipedia <ref>{{Cite pmid|36382883}}</ref> Reevaluation of Piezo1 as a gut RNA sensor #MMPMID36382883 Nickolls AR; O'Brien GS; Shnayder S; Zhang Y; Nagel M; Patapoutian A; Chesler AT Elife 2022[Nov]; 11 (?): ? PMID36382883show ga Piezo1 is a stretch-gated ion channel required for mechanosensation in many organ systems. Recent findings point to a new role for Piezo1 in the gut, suggesting that it is a sensor of microbial single-stranded RNA (ssRNA) rather than mechanical force. If true, this would redefine the scope of Piezo biology. Here, we sought to replicate the central finding that fecal ssRNA is a natural agonist of Piezo1. While we observe that fecal extracts and ssRNA can stimulate calcium influx in certain cell lines, this response is independent of Piezo1. Additionally, sterilized dietary extracts devoid of gut biome RNA show similar cell line-specific stimulatory activity to fecal extracts. Together, our data highlight potential confounds inherent to gut-derived extracts, exclude Piezo1 as a receptor for ssRNA in the gut, and support a dedicated role for Piezo channels in mechanosensing. |*Ion Channels/metabolism[MESH] |*RNA[MESH] |Calcium/metabolism[MESH] |Cell Line[MESH] |Mechanical Phenomena[MESH]Reevaluation of Piezo1 as a gut RNA sensor (epmc).pdf DeepDyve Pubget Overpricing