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10.1016/j.xcrm.2022.100697 http://scihub22266oqcxt.onion/10.1016/j.xcrm.2022.100697 35841887!9247234!35841887     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep+Med 2022 ; 3 (8): 100697 Nephropedia Template TP {{tp|p=35841887|t=2022. Resolving SARS-CoV-2 CD4(+) T cell specificity via reverse epitope discovery |pdf=|usr=}}{{35841887}} gab.com Text Resolving SARS-CoV-2 CD4(+) T cell specificity via reverse epitope discovery JO: Cell Rep Med http://www.kidney.de/pget.php?&tw=1&pmid=35841887 #FOAvip The current strategy to detect immunodominant T cell responses focuses on the antigen, employing large peptide pools to screen for functional cell activation. However, these approaches are labor and sample intensive and scale poorly with increasing size of the pathogen peptidome. T cell receptors (TCRs) recognizing the same epitope frequently have highly similar sequences, and thus, the presence of large sequence similarity clusters in the TCR repertoire likely identify the most public and immunodominant responses. Here, we perform a meta-analysis of large, publicly available single-cell and bulk TCR datasets from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected individuals to identify public CD4(+) responses. We report more than 1,200 alphabetaTCRs forming six prominent similarity clusters and validate histocompatibility leukocyte antigen (HLA) restriction and epitope specificity predictions for five clusters using transgenic T cell lines. Collectively, these data provide information on immunodominant CD4(+) T cell responses to SARS-CoV-2 and demonstrate the utility of the reverse epitope discovery approach. Twit Text FOAVip Resolving SARS-CoV-2 CD4(+) T cell specificity via reverse epitope discovery ????Cell Rep Med http://www.kidney.de/pget.php?&tw=1&pmid=35841887 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35841887 #FOAvip English Wikipedia <ref>{{Cite pmid|35841887}}</ref> Resolving SARS-CoV-2 CD4(+) T cell specificity via reverse epitope discovery #MMPMID35841887 Pogorelyy MV; Rosati E; Minervina AA; Mettelman RC; Scheffold A; Franke A; Bacher P; Thomas PG Cell Rep Med 2022[Aug]; 3 (8): 100697 PMID35841887show ga The current strategy to detect immunodominant T cell responses focuses on the antigen, employing large peptide pools to screen for functional cell activation. However, these approaches are labor and sample intensive and scale poorly with increasing size of the pathogen peptidome. T cell receptors (TCRs) recognizing the same epitope frequently have highly similar sequences, and thus, the presence of large sequence similarity clusters in the TCR repertoire likely identify the most public and immunodominant responses. Here, we perform a meta-analysis of large, publicly available single-cell and bulk TCR datasets from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected individuals to identify public CD4(+) responses. We report more than 1,200 alphabetaTCRs forming six prominent similarity clusters and validate histocompatibility leukocyte antigen (HLA) restriction and epitope specificity predictions for five clusters using transgenic T cell lines. Collectively, these data provide information on immunodominant CD4(+) T cell responses to SARS-CoV-2 and demonstrate the utility of the reverse epitope discovery approach. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |CD4-Positive T-Lymphocytes/chemistry[MESH] |Epitopes/analysis[MESH] |Humans[MESH] |Receptors, Antigen, T-Cell/genetics[MESH]Resolving SARS-CoV-2 CD4(+) T cell specificity via reverse epitope dis(epmc).pdf DeepDyve Pubget Overpricing