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10.1016/j.celrep.2022.111117 http://scihub22266oqcxt.onion/10.1016/j.celrep.2022.111117 35839776!9250890!35839776     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2022 ; 40 (3): 111117 Nephropedia Template TP {{tp|p=35839776|t=2022. Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition |pdf=|usr=}}{{35839776}} gab.com Text Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=35839776 #FOAvip As an enveloped virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) delivers its viral genome into host cells via fusion of the viral and cell membranes. Here, we show that ANO6/TMEM16F-mediated cell surface exposure of phosphatidylserine is critical for SARS-CoV-2 entry and that ANO6-selective inhibitors are effective against SARS-CoV-2 infections. Application of the SARS-CoV-2 Spike pseudotyped virus (SARS2-PsV) evokes a cytosolic Ca(2+) elevation and ANO6-dependent phosphatidylserine externalization in ACE2/TMPRSS2-positive mammalian cells. A high-throughput screening of drug-like chemical libraries identifies three different structural classes of chemicals showing ANO6 inhibitory effects. Among them, A6-001 displays the highest potency and ANO6 selectivity and it inhibits the single-round infection of SARS2-PsV in ACE2/TMPRSS2-positive HEK 293T cells. More importantly, A6-001 strongly inhibits authentic SARS-CoV-2-induced phosphatidylserine scrambling and SARS-CoV-2 viral replications in Vero, Calu-3, and primarily cultured human nasal epithelial cells. These results provide mechanistic insights into the viral entry process and offer a potential target for pharmacological intervention to protect against coronavirus disease 2019 (COVID-19). Twit Text FOAVip Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=35839776 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35839776 #FOAvip English Wikipedia <ref>{{Cite pmid|35839776}}</ref> Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition #MMPMID35839776 Sim JR; Shin DH; Park PG; Park SH; Bae JY; Lee Y; Kang DY; Kim YJ; Aum S; Noh SH; Hwang SJ; Cha HR; Kim CB; Ko SH; Park S; Jeon D; Cho S; Lee GE; Kim J; Moon YH; Kim JO; Nam JS; Kim CH; Moon S; Chung YW; Park MS; Ryu JH; Namkung W; Lee JM; Lee MG Cell Rep 2022[Jul]; 40 (3): 111117 PMID35839776show ga As an enveloped virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) delivers its viral genome into host cells via fusion of the viral and cell membranes. Here, we show that ANO6/TMEM16F-mediated cell surface exposure of phosphatidylserine is critical for SARS-CoV-2 entry and that ANO6-selective inhibitors are effective against SARS-CoV-2 infections. Application of the SARS-CoV-2 Spike pseudotyped virus (SARS2-PsV) evokes a cytosolic Ca(2+) elevation and ANO6-dependent phosphatidylserine externalization in ACE2/TMPRSS2-positive mammalian cells. A high-throughput screening of drug-like chemical libraries identifies three different structural classes of chemicals showing ANO6 inhibitory effects. Among them, A6-001 displays the highest potency and ANO6 selectivity and it inhibits the single-round infection of SARS2-PsV in ACE2/TMPRSS2-positive HEK 293T cells. More importantly, A6-001 strongly inhibits authentic SARS-CoV-2-induced phosphatidylserine scrambling and SARS-CoV-2 viral replications in Vero, Calu-3, and primarily cultured human nasal epithelial cells. These results provide mechanistic insights into the viral entry process and offer a potential target for pharmacological intervention to protect against coronavirus disease 2019 (COVID-19). |*COVID-19 Drug Treatment[MESH] |Angiotensin-Converting Enzyme 2[MESH] |Animals[MESH] |Anoctamins[MESH] |Humans[MESH] |Mammals/metabolism[MESH] |Phosphatidylserines[MESH] |Phospholipid Transfer Proteins/metabolism[MESH] |SARS-CoV-2[MESH]Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase i(epmc).pdf DeepDyve Pubget Overpricing