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10.1002/anie.202204556 http://scihub22266oqcxt.onion/10.1002/anie.202204556 35802496!9350007!35802496     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=35802496&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Angew+Chem+Int+Ed+Engl 2022 ; 61 (38): e202204556 Nephropedia Template TP {{tp|p=35802496|t=2022. Suppression of SARS-CoV-2 Replication with Stabilized and Click-Chemistry Modified siRNAs |pdf=|usr=}}{{35802496}} gab.com Text Suppression of SARS-CoV-2 Replication with Stabilized and Click-Chemistry Modified siRNAs JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=35802496 #FOAvip The emergence of more transmissible or aggressive variants of SARS-CoV-2 requires the development of antiviral medication that is quickly adjustable to evolving viral escape mutations. Here we report the synthesis of chemically stabilized small interfering RNA (siRNA) against SARS-CoV-2. The siRNA can be further modified with receptor ligands such as peptides using Cu(I) -catalysed click-chemistry. We demonstrate that optimized siRNAs can reduce viral loads and virus-induced cytotoxicity by up to five orders of magnitude in cell lines challenged with SARS-CoV-2. Furthermore, we show that an ACE2-binding peptide-conjugated siRNA is able to reduce virus replication and virus-induced apoptosis in 3D mucociliary lung microtissues. The adjustment of the siRNA sequence allows a rapid adaptation of their antiviral activity against different variants of concern. The ability to conjugate the siRNA via click-chemistry to receptor ligands facilitates the construction of targeted siRNAs for a flexible antiviral defence strategy. Twit Text FOAVip Suppression of SARS-CoV-2 Replication with Stabilized and Click-Chemistry Modified siRNAs ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=35802496 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35802496 #FOAvip English Wikipedia <ref>{{Cite pmid|35802496}}</ref> Suppression of SARS-CoV-2 Replication with Stabilized and Click-Chemistry Modified siRNAs #MMPMID35802496 Traube FR; Stern M; Tolke AJ; Rudelius M; Mejias-Perez E; Raddaoui N; Kummerer BM; Douat C; Streshnev F; Albanese M; Wratil PR; Gartner YV; Nainyte M; Giorgio G; Michalakis S; Schneider S; Streeck H; Muller M; Keppler OT; Carell T Angew Chem Int Ed Engl 2022[Sep]; 61 (38): e202204556 PMID35802496show ga The emergence of more transmissible or aggressive variants of SARS-CoV-2 requires the development of antiviral medication that is quickly adjustable to evolving viral escape mutations. Here we report the synthesis of chemically stabilized small interfering RNA (siRNA) against SARS-CoV-2. The siRNA can be further modified with receptor ligands such as peptides using Cu(I) -catalysed click-chemistry. We demonstrate that optimized siRNAs can reduce viral loads and virus-induced cytotoxicity by up to five orders of magnitude in cell lines challenged with SARS-CoV-2. Furthermore, we show that an ACE2-binding peptide-conjugated siRNA is able to reduce virus replication and virus-induced apoptosis in 3D mucociliary lung microtissues. The adjustment of the siRNA sequence allows a rapid adaptation of their antiviral activity against different variants of concern. The ability to conjugate the siRNA via click-chemistry to receptor ligands facilitates the construction of targeted siRNAs for a flexible antiviral defence strategy. |*COVID-19[MESH] |*SARS-CoV-2/genetics[MESH] |Antiviral Agents/pharmacology[MESH] |Humans[MESH] |Ligands[MESH] |RNA, Small Interfering/pharmacology[MESH]Suppression of SARS-CoV-2 Replication with Stabilized and Click-Chemis(epmc).pdf DeepDyve Pubget Overpricing