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10.1016/j.prp.2022.154000

http://scihub22266oqcxt.onion/10.1016/j.prp.2022.154000
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35797854!9245394!35797854
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suck abstract from ncbi


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pmid35797854      Pathol+Res+Pract 2022 ; 236 (ä): 154000
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  • Cell tropism and viral clearance during SARS-CoV-2 lung infection #MMPMID35797854
  • Schwab C; Domke LM; Rose F; Hausser I; Schirmacher P; Longerich T
  • Pathol Res Pract 2022[Aug]; 236 (ä): 154000 PMID35797854show ga
  • Pulmonary capillary microthrombosis has been proposed as a major pathogenetic factor driving severe COVID-19. Autopsy studies reported endothelialitis but it is under debate if it is caused by SARS-CoV-2 infection of endothelial cells. In this study, RNA in situ hybridization was used to detect viral RNA and to identify the infected cell types in lung tissue of 40 patients with fatal COVID-19. SARS-CoV-2 Spike protein-coding RNA showed a steadily decreasing signal abundance over a period of three weeks. Besides the original virus strain the variants of concern Alpha (B.1.1.7), Delta (B.1.617.2), and Omicron (B.1.1.529) could also be detected by the assay. Viral RNA was mainly detected in alveolar macrophages and pulmonary epithelial cells, while only single virus-positive endothelial cells were observed even in cases with high viral load suggesting that viral infection of endothelial cells is not a key factor for the development of pulmonary capillary microthrombosis.
  • |*COVID-19[MESH]
  • |*Thrombosis/pathology[MESH]
  • |Endothelial Cells/metabolism[MESH]
  • |Humans[MESH]
  • |Lung/pathology[MESH]
  • |RNA, Viral[MESH]
  • |SARS-CoV-2[MESH]
  • |Spike Glycoprotein, Coronavirus[MESH]


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