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10.1016/j.ebiom.2022.104109 http://scihub22266oqcxt.onion/10.1016/j.ebiom.2022.104109 35752106!9240806!35752106     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=35752106&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       EBioMedicine 2022 ; 81 (ä): 104109 Nephropedia Template TP {{tp|p=35752106|t=2022. Immunoassay for quantification of antigen-specific IgG fucosylation |pdf=|usr=}}{{35752106}} gab.com Text Immunoassay for quantification of antigen-specific IgG fucosylation JO: EBioMedicine http://www.kidney.de/pget.php?&tw=1&pmid=35752106 #FOAvip BACKGROUND: Immunoglobulin G (IgG) antibodies serve a crucial immuno-protective function mediated by IgG Fc receptors (FcgammaR). Absence of fucose on the highly conserved N-linked glycan in the IgG Fc domain strongly enhances IgG binding and activation of myeloid and natural killer (NK) cell FcgammaRs. Although afucosylated IgG can provide increased protection (malaria and HIV), it also boosts immunopathologies in alloimmune diseases, COVID-19 and dengue fever. Quantifying IgG fucosylation currently requires sophisticated methods such as liquid chromatography-mass spectrometry (LC-MS) and extensive analytical skills reserved to highly specialized laboratories. METHODS: Here, we introduce the Fucose-sensitive Enzyme-linked immunosorbent assay (ELISA) for Antigen-Specific IgG (FEASI), an immunoassay capable of simultaneously quantitating and qualitatively determining IgG responses. FEASI is a two-tier immunoassay; the first assay is used to quantify antigen-specific IgG (IgG ELISA), while the second gives FcgammaRIIIa binding-dependent readout which is highly sensitive to both the IgG quantity and the IgG Fc fucosylation (FcgammaR-IgG ELISA). FINDINGS: IgG Fc fucosylation levels, independently determined by LC-MS and FEASI, in COVID-19 responses to the spike (S) antigen, correlated very strongly by simple linear regression (R(2)=0.93, p < 0.0001). The FEASI method was then used to quantify IgG levels and fucosylation in COVID-19 convalescent plasma which was independently validated by LC-MS. INTERPRETATION: FEASI can be reliably implemented to measure relative and absolute IgG Fc fucosylation and quantify binding of antigen-specific IgG to FcgammaR in a high-throughput manner accessible to all diagnostic and research laboratories. FUNDING: This work was funded by the Stichting Sanquin Bloedvoorziening (PPOC 19-08 and SQI00041) and ZonMW 10430 01 201 0021. Twit Text FOAVip Immunoassay for quantification of antigen-specific IgG fucosylation ????EBioMedicine http://www.kidney.de/pget.php?&tw=1&pmid=35752106 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35752106 #FOAvip English Wikipedia <ref>{{Cite pmid|35752106}}</ref> Immunoassay for quantification of antigen-specific IgG fucosylation #MMPMID35752106 Sustic T; Van Coillie J; Larsen MD; Derksen NIL; Szittner Z; Nouta J; Wang W; Damelang T; Rebergen I; Linty F; Visser R; Mok JY; Geerdes DM; van Esch WJE; de Taeye SW; van Gils MJ; van de Watering L; van der Schoot CE; Wuhrer M; Rispens T; Vidarsson G EBioMedicine 2022[Jul]; 81 (ä): 104109 PMID35752106show ga BACKGROUND: Immunoglobulin G (IgG) antibodies serve a crucial immuno-protective function mediated by IgG Fc receptors (FcgammaR). Absence of fucose on the highly conserved N-linked glycan in the IgG Fc domain strongly enhances IgG binding and activation of myeloid and natural killer (NK) cell FcgammaRs. Although afucosylated IgG can provide increased protection (malaria and HIV), it also boosts immunopathologies in alloimmune diseases, COVID-19 and dengue fever. Quantifying IgG fucosylation currently requires sophisticated methods such as liquid chromatography-mass spectrometry (LC-MS) and extensive analytical skills reserved to highly specialized laboratories. METHODS: Here, we introduce the Fucose-sensitive Enzyme-linked immunosorbent assay (ELISA) for Antigen-Specific IgG (FEASI), an immunoassay capable of simultaneously quantitating and qualitatively determining IgG responses. FEASI is a two-tier immunoassay; the first assay is used to quantify antigen-specific IgG (IgG ELISA), while the second gives FcgammaRIIIa binding-dependent readout which is highly sensitive to both the IgG quantity and the IgG Fc fucosylation (FcgammaR-IgG ELISA). FINDINGS: IgG Fc fucosylation levels, independently determined by LC-MS and FEASI, in COVID-19 responses to the spike (S) antigen, correlated very strongly by simple linear regression (R(2)=0.93, p < 0.0001). The FEASI method was then used to quantify IgG levels and fucosylation in COVID-19 convalescent plasma which was independently validated by LC-MS. INTERPRETATION: FEASI can be reliably implemented to measure relative and absolute IgG Fc fucosylation and quantify binding of antigen-specific IgG to FcgammaR in a high-throughput manner accessible to all diagnostic and research laboratories. FUNDING: This work was funded by the Stichting Sanquin Bloedvoorziening (PPOC 19-08 and SQI00041) and ZonMW 10430 01 201 0021. |*Fucose/chemistry/metabolism[MESH] |*Immunoglobulin G/chemistry[MESH] |*Receptors, IgG/chemistry[MESH] |COVID-19 Serotherapy[MESH] |COVID-19/diagnosis/therapy[MESH] |Enzyme-Linked Immunosorbent Assay/methods[MESH] |Humans[MESH] |Immunization, Passive[MESH]Immunoassay for quantification of antigen-specific IgG fucosylation (epmc).pdf DeepDyve Pubget Overpricing