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10.3390/ijms23126587 http://scihub22266oqcxt.onion/10.3390/ijms23126587 35743031!9223907!35743031     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2022 ; 23 (12): ä Nephropedia Template TP {{tp|p=35743031|t=2022. Human Superantibodies to 3CL(pro) Inhibit Replication of SARS-CoV-2 across Variants |pdf=|usr=}}{{35743031}} gab.com Text Human Superantibodies to 3CL(pro) Inhibit Replication of SARS-CoV-2 across Variants JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=35743031 #FOAvip Broadly effective and safe anti-coronavirus agent is existentially needed. Major protease (3CL(pro)) is a highly conserved enzyme of betacoronaviruses. The enzyme plays pivotal role in the virus replication cycle. Thus, it is a good target of a broadly effective anti-Betacoronavirus agent. In this study, human single-chain antibodies (HuscFvs) of the SARS-CoV-2 3CL(pro) were generated using phage display technology. The 3CL(pro)-bound phages were used to infect Escherichia coli host for the production the 3CL(pro)-bound HuscFvs. Computerized simulation was used to guide the selection of the phage infected-E. coli clones that produced HuscFvs with the 3CL(pro) inhibitory potential. HuscFvs of three phage infected-E. coli clones were predicted to form contact interface with residues for 3CL(pro) catalytic activity, substrate binding, and homodimerization. These HuscFvs were linked to a cell-penetrating peptide to make them cell-penetrable, i.e., became superantibodies. The superantibodies blocked the 3CL(pro) activity in vitro, were not toxic to human cells, traversed across membrane of 3CL(pro)-expressing cells to co-localize with the intracellular 3CL(pro) and most of all, they inhibited replication of authentic SARS-CoV-2 Wuhan wild type and alpha, beta, delta, and Omicron variants that were tested. The superantibodies should be investigated further towards clinical application as a safe and broadly effective anti-Betacoronavirus agent. Twit Text FOAVip Human Superantibodies to 3CL(pro) Inhibit Replication of SARS-CoV-2 across Variants ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=35743031 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35743031 #FOAvip English Wikipedia <ref>{{Cite pmid|35743031}}</ref> Human Superantibodies to 3CL(pro) Inhibit Replication of SARS-CoV-2 across Variants #MMPMID35743031 Glab-Ampai K; Kaewchim K; Saenlom T; Thepsawat W; Mahasongkram K; Sookrung N; Chaicumpa W; Chulanetra M Int J Mol Sci 2022[Jun]; 23 (12): ä PMID35743031show ga Broadly effective and safe anti-coronavirus agent is existentially needed. Major protease (3CL(pro)) is a highly conserved enzyme of betacoronaviruses. The enzyme plays pivotal role in the virus replication cycle. Thus, it is a good target of a broadly effective anti-Betacoronavirus agent. In this study, human single-chain antibodies (HuscFvs) of the SARS-CoV-2 3CL(pro) were generated using phage display technology. The 3CL(pro)-bound phages were used to infect Escherichia coli host for the production the 3CL(pro)-bound HuscFvs. Computerized simulation was used to guide the selection of the phage infected-E. coli clones that produced HuscFvs with the 3CL(pro) inhibitory potential. HuscFvs of three phage infected-E. coli clones were predicted to form contact interface with residues for 3CL(pro) catalytic activity, substrate binding, and homodimerization. These HuscFvs were linked to a cell-penetrating peptide to make them cell-penetrable, i.e., became superantibodies. The superantibodies blocked the 3CL(pro) activity in vitro, were not toxic to human cells, traversed across membrane of 3CL(pro)-expressing cells to co-localize with the intracellular 3CL(pro) and most of all, they inhibited replication of authentic SARS-CoV-2 Wuhan wild type and alpha, beta, delta, and Omicron variants that were tested. The superantibodies should be investigated further towards clinical application as a safe and broadly effective anti-Betacoronavirus agent. |*COVID-19 Drug Treatment[MESH] |*SARS-CoV-2[MESH] |Coronavirus 3C Proteases[MESH] |Escherichia coli[MESH] |Humans[MESH]Human Superantibodies to 3CL(pro) Inhibit Replication of SARS-CoV-2 ac(epmc).pdf DeepDyve Pubget Overpricing