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10.1007/s00203-022-03029-y

http://scihub22266oqcxt.onion/10.1007/s00203-022-03029-y
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35737122!9223264!35737122
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suck abstract from ncbi


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pmid35737122      Arch+Microbiol 2022 ; 204 (7): 415
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  • Current RT-qPCR to detect SARS-CoV-2 may give positive results for related coronaviruses #MMPMID35737122
  • Martinez-Murcia A; Garcia-Sirera A; Navarro A; Perez L
  • Arch Microbiol 2022[Jun]; 204 (7): 415 PMID35737122show ga
  • Some weeks after the first CoVID-19 outbreak, the World Health Organization published some real-time PCR (qPCR) protocols developed by different health reference centers. These qPCR designs are being used worldwide to detect SARS-CoV-2 in the population, to monitor the prevalence of the virus during the pandemic. Moreover, some of these protocols to detect SARS-CoV-2 have widely been applied to environmental samples for epidemiological surveillance purposes. In the present work, the specificity of these currently used RT-qPCR designs was validated in vitro using SARS-CoV-2 and highly related coronaviral genomic sequences and compared to performance of the commercially available GPS CoVID-19 dtec-RT-qPCR Test. Assays performed with SARS-CoV-2-related genomes showed positive amplification when using some of these qPCR methods, indicating they may give SARS-CoV-2 false positives. This finding may be particularly relevant for SARS-CoV-2 monitoring of environmental samples, where an unknown pool of phylogenetically close-related viruses may exist.
  • |*COVID-19/diagnosis[MESH]
  • |*SARS-CoV-2/genetics[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]
  • |Real-Time Polymerase Chain Reaction/methods[MESH]


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