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10.1016/j.eti.2022.102715

http://scihub22266oqcxt.onion/10.1016/j.eti.2022.102715
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suck abstract from ncbi


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pmid35694201      Environ+Technol+Innov 2022 ; 27 (ä): 102715
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  • Inactivation of porcine epidemic diarrhea virus with electron beam irradiation under cold chain conditions #MMPMID35694201
  • Liu Y; Shao Y; Wang L; Lu W; Li S; Xu D; Fu YV
  • Environ Technol Innov 2022[Aug]; 27 (ä): 102715 PMID35694201show ga
  • The many instances of COVID-19 outbreaks suggest that cold chains are a possible route for the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, owing to the low temperatures of cold chains, which are normally below 0 degrees C, there are limited options for virus inactivation. Here, high-energy electron beam (E-beam) irradiation was used to inactivate porcine epidemic diarrhea virus (PEDV) under simulated cold chain conditions. This coronavirus was used as a surrogate for SARS-CoV-2. The possible mechanism by which high-energy E-beam irradiation inactivates PEDV was also explored. An irradiation dose of 10 kGy reduced the PEDV infectious viral titer by 1.68-1.76 log(10)TCID 50/100 mu L in the cold chain environment, suggesting that greater than 98.1% of PEDV was inactivated. E-beam irradiation at 5-30 kGy damaged the viral genomic RNA with an efficiency of 46.25%-92.11%. The integrity of the viral capsid was disrupted at 20 kGy. The rapid and effective inactivation of PEDV at temperatures below freezing indicates high-energy E-beam irradiation as a promising technology for disinfecting SARS-CoV-2 in cold chain logistics to limit the transmission of COVID-19.
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