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10.1016/j.xpro.2022.101406 http://scihub22266oqcxt.onion/10.1016/j.xpro.2022.101406 35611119!9057979!35611119     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2022 ; 3 (2): 101406 Nephropedia Template TP {{tp|p=35611119|t=2022. An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2 |pdf=|usr=}}{{35611119}} gab.com Text An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2 JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=35611119 #FOAvip Elucidating the molecular interactions between virus and host is fundamental to understanding the mechanism of viral pathogenesis. Here, we present a protocol to screen SARS-CoV-2 protein interactors using an antibody-based TurboID proximity labeling approach. This technique directly identifies biotinylated peptides labeled by the TurboID-tagged viral proteins. We describe the steps to prepare biotinylated peptide samples for mass spectrometry analysis and a stringent workflow to identify biotinylated high-confidence interactors of the virus by filtering out non-specific co-purified proteins. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022). Twit Text FOAVip An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2 ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=35611119 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35611119 #FOAvip English Wikipedia <ref>{{Cite pmid|35611119}}</ref> An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2 #MMPMID35611119 Shang L; Zhang Y; Liu Y; Jin C; Zhao Y; Zhang J; Wang PH; Wang J STAR Protoc 2022[Jun]; 3 (2): 101406 PMID35611119show ga Elucidating the molecular interactions between virus and host is fundamental to understanding the mechanism of viral pathogenesis. Here, we present a protocol to screen SARS-CoV-2 protein interactors using an antibody-based TurboID proximity labeling approach. This technique directly identifies biotinylated peptides labeled by the TurboID-tagged viral proteins. We describe the steps to prepare biotinylated peptide samples for mass spectrometry analysis and a stringent workflow to identify biotinylated high-confidence interactors of the virus by filtering out non-specific co-purified proteins. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022). |*COVID-19/diagnosis[MESH] |*SARS-CoV-2[MESH] |Antibodies[MESH] |Humans[MESH] |Mass Spectrometry[MESH]An antibody-based proximity labeling protocol to identify biotinylated(epmc).pdf DeepDyve Pubget Overpricing