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10.1038/s41467-022-30134-9 http://scihub22266oqcxt.onion/10.1038/s41467-022-30134-9 35508460!9068693!35508460     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=35508460&cmd=llinks): Failed to open stream: HTTP request failed! 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Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen |pdf=|usr=}}{{35508460}} gab.com Text Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=35508460 #FOAvip Interferon restricts SARS-CoV-2 replication in cell culture, but only a handful of Interferon Stimulated Genes with antiviral activity against SARS-CoV-2 have been identified. Here, we describe a functional CRISPR/Cas9 screen aiming at identifying SARS-CoV-2 restriction factors. We identify DAXX, a scaffold protein residing in PML nuclear bodies known to limit the replication of DNA viruses and retroviruses, as a potent inhibitor of SARS-CoV-2 and SARS-CoV replication in human cells. Basal expression of DAXX is sufficient to limit the replication of SARS-CoV-2, and DAXX over-expression further restricts infection. DAXX restricts an early, post-entry step of the SARS-CoV-2 life cycle. DAXX-mediated restriction of SARS-CoV-2 is independent of the SUMOylation pathway but dependent on its D/E domain, also necessary for its protein-folding activity. SARS-CoV-2 infection triggers the re-localization of DAXX to cytoplasmic sites and promotes its degradation. Mechanistically, this process is mediated by the viral papain-like protease (PLpro) and the proteasome. Together, these results demonstrate that DAXX restricts SARS-CoV-2, which in turn has evolved a mechanism to counteract its action. Twit Text FOAVip Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=35508460 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35508460 #FOAvip English Wikipedia <ref>{{Cite pmid|35508460}}</ref> Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen #MMPMID35508460 Mac Kain A; Maarifi G; Aicher SM; Arhel N; Baidaliuk A; Munier S; Donati F; Vallet T; Tran QD; Hardy A; Chazal M; Porrot F; OhAinle M; Carlson-Stevermer J; Oki J; Holden K; Zimmer G; Simon-Loriere E; Bruel T; Schwartz O; van der Werf S; Jouvenet N; Nisole S; Vignuzzi M; Roesch F Nat Commun 2022[May]; 13 (1): 2442 PMID35508460show ga Interferon restricts SARS-CoV-2 replication in cell culture, but only a handful of Interferon Stimulated Genes with antiviral activity against SARS-CoV-2 have been identified. Here, we describe a functional CRISPR/Cas9 screen aiming at identifying SARS-CoV-2 restriction factors. We identify DAXX, a scaffold protein residing in PML nuclear bodies known to limit the replication of DNA viruses and retroviruses, as a potent inhibitor of SARS-CoV-2 and SARS-CoV replication in human cells. Basal expression of DAXX is sufficient to limit the replication of SARS-CoV-2, and DAXX over-expression further restricts infection. DAXX restricts an early, post-entry step of the SARS-CoV-2 life cycle. DAXX-mediated restriction of SARS-CoV-2 is independent of the SUMOylation pathway but dependent on its D/E domain, also necessary for its protein-folding activity. SARS-CoV-2 infection triggers the re-localization of DAXX to cytoplasmic sites and promotes its degradation. Mechanistically, this process is mediated by the viral papain-like protease (PLpro) and the proteasome. Together, these results demonstrate that DAXX restricts SARS-CoV-2, which in turn has evolved a mechanism to counteract its action. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |CRISPR-Cas Systems[MESH] |Co-Repressor Proteins/genetics/metabolism[MESH] |Humans[MESH] |Interferons/metabolism[MESH] |Molecular Chaperones/genetics/metabolism[MESH]Identification of DAXX as a restriction factor of SARS-CoV-2 through a(epmc).pdf DeepDyve Pubget Overpricing