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10.3390/v14040705 http://scihub22266oqcxt.onion/10.3390/v14040705 35458435!9032829!35458435     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2022 ; 14 (4): ä Nephropedia Template TP {{tp|p=35458435|t=2022. SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an alpha(v)beta(3) Integrin-Mediated Endocytosis Even in the Presence of Vaccine-Elicited Neutralizing Antibodies |pdf=|usr=}}{{35458435}} gab.com Text SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an alpha(v)beta(3) Integrin-Mediated Endocytosis Even in the Presence of Vaccine-Elicited Neutralizing Antibodies JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=35458435 #FOAvip Integrins represent a gateway of entry for many viruses and the Arg-Gly-Asp (RGD) motif is the smallest sequence necessary for proteins to bind integrins. All Severe Acute Respiratory Syndrome Virus type 2 (SARS-CoV-2) lineages own an RGD motif (aa 403-405) in their receptor binding domain (RBD). We recently showed that SARS-CoV-2 gains access into primary human lung microvascular endothelial cells (HL-mECs) lacking Angiotensin-converting enzyme 2 (ACE2) expression through this conserved RGD motif. Following its entry, SARS-CoV-2 remodels cell phenotype and promotes angiogenesis in the absence of productive viral replication. Here, we highlight the alpha(v)beta(3) integrin as the main molecule responsible for SARS-CoV-2 infection of HL-mECs via a clathrin-dependent endocytosis. Indeed, pretreatment of virus with alpha(v)beta(3) integrin or pretreatment of cells with a monoclonal antibody against alpha(v)beta(3) integrin was found to inhibit SARS-CoV-2 entry into HL-mECs. Surprisingly, the anti-Spike antibodies evoked by vaccination were neither able to impair Spike/integrin interaction nor to prevent SARS-CoV-2 entry into HL-mECs. Our data highlight the RGD motif in the Spike protein as a functional constraint aimed to maintain the interaction of the viral envelope with integrins. At the same time, our evidences call for the need of intervention strategies aimed to neutralize the SARS-CoV-2 integrin-mediated infection of ACE2-negative cells in the vaccine era. Twit Text FOAVip SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an alpha(v)beta(3) Integrin-Mediated Endocytosis Even in the Presence of Vaccine-Elicited Neutralizing Antibodies ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=35458435 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35458435 #FOAvip English Wikipedia <ref>{{Cite pmid|35458435}}</ref> SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an alpha(v)beta(3) Integrin-Mediated Endocytosis Even in the Presence of Vaccine-Elicited Neutralizing Antibodies #MMPMID35458435 Bugatti A; Filippini F; Bardelli M; Zani A; Chiodelli P; Messali S; Caruso A; Caccuri F Viruses 2022[Mar]; 14 (4): ä PMID35458435show ga Integrins represent a gateway of entry for many viruses and the Arg-Gly-Asp (RGD) motif is the smallest sequence necessary for proteins to bind integrins. All Severe Acute Respiratory Syndrome Virus type 2 (SARS-CoV-2) lineages own an RGD motif (aa 403-405) in their receptor binding domain (RBD). We recently showed that SARS-CoV-2 gains access into primary human lung microvascular endothelial cells (HL-mECs) lacking Angiotensin-converting enzyme 2 (ACE2) expression through this conserved RGD motif. Following its entry, SARS-CoV-2 remodels cell phenotype and promotes angiogenesis in the absence of productive viral replication. Here, we highlight the alpha(v)beta(3) integrin as the main molecule responsible for SARS-CoV-2 infection of HL-mECs via a clathrin-dependent endocytosis. Indeed, pretreatment of virus with alpha(v)beta(3) integrin or pretreatment of cells with a monoclonal antibody against alpha(v)beta(3) integrin was found to inhibit SARS-CoV-2 entry into HL-mECs. Surprisingly, the anti-Spike antibodies evoked by vaccination were neither able to impair Spike/integrin interaction nor to prevent SARS-CoV-2 entry into HL-mECs. Our data highlight the RGD motif in the Spike protein as a functional constraint aimed to maintain the interaction of the viral envelope with integrins. At the same time, our evidences call for the need of intervention strategies aimed to neutralize the SARS-CoV-2 integrin-mediated infection of ACE2-negative cells in the vaccine era. |*COVID-19/prevention & control[MESH] |*Vaccines[MESH] |Angiotensin-Converting Enzyme 2[MESH] |Antibodies, Neutralizing[MESH] |Endocytosis[MESH] |Endothelial Cells/metabolism[MESH] |Humans[MESH] |Integrin alphaV/metabolism[MESH] |Integrin beta3/metabolism[MESH] |Oligopeptides[MESH] |Protein Binding[MESH] |SARS-CoV-2[MESH]SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an al(epmc).pdf DeepDyve Pubget Overpricing