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10.1002/anie.202202008 http://scihub22266oqcxt.onion/10.1002/anie.202202008 35451177!ä!35451177 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Angew+Chem+Int+Ed+Engl 2022 ; 61 (27): e202202008 Nephropedia Template TP {{tp|p=35451177|t=2022. Photocatalytic Chemical Crosslinking for Profiling RNA-Protein Interactions in Living Cells |pdf=|usr=}}{{35451177}} gab.com Text Photocatalytic Chemical Crosslinking for Profiling RNA-Protein Interactions in Living Cells JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=35451177 #FOAvip The dynamic interactions between RNAs and proteins play crucial roles in regulating diverse cellular processes. Proteome-wide characterization of these interactions in their native cellular context remains desirable but challenging. Herein, we developed a photocatalytic crosslinking (PhotoCAX) strategy coupled with mass spectrometry (PhotoCAX-MS) and RNA sequencing (PhotoCAX-seq) for the study of the composition and dynamics of protein-RNA interactions. By integrating the blue light-triggered photocatalyst with a dual-functional RNA-protein crosslinker (RP-linker) and the phase separation-based enrichment strategy, PhotoCAX-MS revealed a total of 2044 RBPs in human HEK293 cells. We further employed PhotoCAX to investigate the dynamic change of RBPome in macrophage cells upon LPS-stimulation, as well as the identification of RBPs interacting directly with the 5' untranslated regions of SARS-CoV-2 RNA. Twit Text FOAVip Photocatalytic Chemical Crosslinking for Profiling RNA-Protein Interactions in Living Cells ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=35451177 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35451177 #FOAvip English Wikipedia <ref>{{Cite pmid|35451177}}</ref> Photocatalytic Chemical Crosslinking for Profiling RNA-Protein Interactions in Living Cells #MMPMID35451177 Luo H; Tang W; Liu H; Zeng X; Ngai WSC; Gao R; Li H; Li R; Zheng H; Guo J; Qin F; Wang G; Li K; Fan X; Zou P; Chen PR Angew Chem Int Ed Engl 2022[Jul]; 61 (27): e202202008 PMID35451177show ga The dynamic interactions between RNAs and proteins play crucial roles in regulating diverse cellular processes. Proteome-wide characterization of these interactions in their native cellular context remains desirable but challenging. Herein, we developed a photocatalytic crosslinking (PhotoCAX) strategy coupled with mass spectrometry (PhotoCAX-MS) and RNA sequencing (PhotoCAX-seq) for the study of the composition and dynamics of protein-RNA interactions. By integrating the blue light-triggered photocatalyst with a dual-functional RNA-protein crosslinker (RP-linker) and the phase separation-based enrichment strategy, PhotoCAX-MS revealed a total of 2044 RBPs in human HEK293 cells. We further employed PhotoCAX to investigate the dynamic change of RBPome in macrophage cells upon LPS-stimulation, as well as the identification of RBPs interacting directly with the 5' untranslated regions of SARS-CoV-2 RNA. |*COVID-19[MESH] |*RNA-Binding Proteins/metabolism[MESH] |Cell Survival[MESH] |HEK293 Cells[MESH] |Humans[MESH] |RNA, Viral[MESH]Photocatalytic Chemical Crosslinking for Profiling RNA-Protein Interac(epmc).pdf DeepDyve Pubget Overpricing