Aurich K; Wesche J; Ulbricht M; Otto O; Greinacher A; Palankar R
Sci Rep 2022[Apr]; 12 (1): 6229 PMID35422472show ga
Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca(2+) release from storage organelles and higher activity of Ca(2+) pumps at temperatures < 15 degrees C triggers cytoskeleton changes. This is suppressed by Mg(2+) addition, avoiding a shift in Ca(2+) hemostasis and cytoskeletal alterations. We report on the impact of 2-10 mM Mg(2+) on cytoskeleton alterations of platelets from PC stored at room temperature (RT) or 4 degrees C in additive solution (PAS), 30% plasma. Deformation of platelets was assessed by real-time deformability cytometry (RT-DC), a method for biomechanical cell characterization. Deformation was strongly affected by storage at 4 degrees C and preserved by Mg(2+) addition >/= 4 mM Mg(2+) (mean +/- SD of median deformation 4 degrees C vs. 4 degrees C + 10 mM Mg(2+) 0.073 +/- 0.021 vs. 0.118 +/- 0.023, p < 0.01; n = 6, day 7). These results were confirmed by immunofluorescence microscopy, showing that Mg(2+) >/= 4 mM prevents 4 degrees C storage induced cytoskeletal structure lesion. Standard in vitro platelet function tests showed minor differences between RT and cold-stored platelets. Hypotonic shock response was not significantly different between RT stored (56.38 +/- 29.36%) and cold-stored platelets with (55.22 +/- 11.16%) or without magnesium (45.65 +/- 11.59%; p = 0.042, all n = 6, day 1). CD62P expression and platelet aggregation response were similar between RT and 4 degrees C stored platelets, with minor changes in the presence of higher Mg(2+) concentrations. In conclusion, increasing Mg(2+) up to 10 mM in PAS counteracts 4 degrees C storage lesions in platelets, maintains platelet cytoskeletal integrity and biomechanical properties comparable to RT stored platelets.
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Sci+Rep 2022 ; 12 (1): 6229
