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10.1038/s41467-022-29255-y http://scihub22266oqcxt.onion/10.1038/s41467-022-29255-y 35338134!8956608!35338134     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2022 ; 13 (1): 1609 Nephropedia Template TP {{tp|p=35338134|t=2022. Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium |pdf=|usr=}}{{35338134}} gab.com Text Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=35338134 #FOAvip Ultrastructural studies of SARS-CoV-2 infected cells are crucial to better understand the mechanisms of viral entry and budding within host cells. Here, we examined human airway epithelium infected with three different isolates of SARS-CoV-2 including the B.1.1.7 variant by transmission electron microscopy and tomography. For all isolates, the virus infected ciliated but not goblet epithelial cells. Key SARS-CoV-2 entry molecules, ACE2 and TMPRSS2, were found to be localised to the plasma membrane including microvilli but excluded from cilia. Consistently, extracellular virions were seen associated with microvilli and the apical plasma membrane but rarely with ciliary membranes. Profiles indicative of viral fusion where tomography showed that the viral membrane was continuous with the apical plasma membrane and the nucleocapsids diluted, compared with unfused virus, demonstrate that the plasma membrane is one site of entry where direct fusion releasing the nucleoprotein-encapsidated genome occurs. Intact intracellular virions were found within ciliated cells in compartments with a single membrane bearing S glycoprotein. Tomography showed concentration of nucleocapsids round the periphery of profiles strongly suggestive of viral budding into these compartments and this may explain how virions gain their S glycoprotein containing envelope. Twit Text FOAVip Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=35338134 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35338134 #FOAvip English Wikipedia <ref>{{Cite pmid|35338134}}</ref> Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium #MMPMID35338134 Pinto AL; Rai RK; Brown JC; Griffin P; Edgar JR; Shah A; Singanayagam A; Hogg C; Barclay WS; Futter CE; Burgoyne T Nat Commun 2022[Mar]; 13 (1): 1609 PMID35338134show ga Ultrastructural studies of SARS-CoV-2 infected cells are crucial to better understand the mechanisms of viral entry and budding within host cells. Here, we examined human airway epithelium infected with three different isolates of SARS-CoV-2 including the B.1.1.7 variant by transmission electron microscopy and tomography. For all isolates, the virus infected ciliated but not goblet epithelial cells. Key SARS-CoV-2 entry molecules, ACE2 and TMPRSS2, were found to be localised to the plasma membrane including microvilli but excluded from cilia. Consistently, extracellular virions were seen associated with microvilli and the apical plasma membrane but rarely with ciliary membranes. Profiles indicative of viral fusion where tomography showed that the viral membrane was continuous with the apical plasma membrane and the nucleocapsids diluted, compared with unfused virus, demonstrate that the plasma membrane is one site of entry where direct fusion releasing the nucleoprotein-encapsidated genome occurs. Intact intracellular virions were found within ciliated cells in compartments with a single membrane bearing S glycoprotein. Tomography showed concentration of nucleocapsids round the periphery of profiles strongly suggestive of viral budding into these compartments and this may explain how virions gain their S glycoprotein containing envelope. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Epithelium/metabolism[MESH] |Humans[MESH]Ultrastructural insight into SARS-CoV-2 entry and budding in human air(epmc).pdf DeepDyve Pubget Overpricing