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10.1007/s11262-022-01893-w

http://scihub22266oqcxt.onion/10.1007/s11262-022-01893-w
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35301621!ä!35301621

suck abstract from ncbi


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pmid35301621      Virus+Genes 2022 ; 58 (3): 203-213
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  • Comparative trachea transcriptome analysis in SPF broiler chickens infected with avian infectious bronchitis and avian influenza viruses #MMPMID35301621
  • Zamzam SH; Ghalyanchilangeroudi A; Khosravi AR
  • Virus Genes 2022[Jun]; 58 (3): 203-213 PMID35301621show ga
  • Infectious bronchitis virus (IBV) and avian influenza virus (AIV) are two major respiratory infections in chickens. The coinfection of these viruses can cause significant financial losses and severe complications in the poultry industry across the world. To examine transcriptome profile changes during the early stages of infection, differential transcriptional profiles in tracheal tissue of three infected groups (i.e., IBV, AIV, and coinfected) were compared with the control group. Specific-pathogen-free chickens were challenged with Iranian variant-2-like IBV (IS/1494), UT-Barin isolates of H9N2 (A/chicken/Mashhad/UT-Barin/2017), and IBV-AIV coinfection; then, RNA was extracted from tracheal tissue. The Illumina RNA-sequencing (RNA-seq) technique was employed to investigate changes in the Transcriptome. Up- and downregulated differentially expressed genes (DEGs) were detected in the trachea transcriptome of all groups. The Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology databases were examined to identify possible relationships between DEGs. In the experimental groups, upregulated genes were higher compared to downregulated genes. A more severe immune response was observed in the coinfected group; further, cytokine-cytokine receptor interaction, RIG-I-like receptor signaling, Toll-like receptor signaling, NOD-like receptor signaling, Janus kinase/signal transducer, and activator of transcription, and apoptotic pathways were important upregulated genes in this group. The findings of this paper may give a better understanding of transcriptome changes in the trachea during the early stages of infection with these viruses.
  • |*Bronchitis/genetics/veterinary[MESH]
  • |*Coinfection[MESH]
  • |*Coronavirus Infections[MESH]
  • |*Infectious bronchitis virus/genetics[MESH]
  • |*Influenza A Virus, H9N2 Subtype/genetics[MESH]
  • |*Influenza in Birds/genetics[MESH]
  • |*Poultry Diseases/genetics[MESH]
  • |Animals[MESH]
  • |Chickens[MESH]
  • |Gene Expression Profiling[MESH]
  • |Iran[MESH]
  • |RNA[MESH]
  • |Trachea[MESH]


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