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10.7554/eLife.71945 http://scihub22266oqcxt.onion/10.7554/eLife.71945 35293857!9054133!35293857     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\35293857.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Elife 2022 ; 11 (ä): ä Nephropedia Template TP {{tp|p=35293857|t=2022. SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome |pdf=|usr=}}{{35293857}} gab.com Text SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=35293857 #FOAvip Viral infection involves complex set of events orchestrated by multiple viral proteins. To identify functions of SARS-CoV-2 proteins, we performed transcriptomic analyses of cells expressing individual viral proteins. Expression of Nsp14, a protein involved in viral RNA replication, provoked a dramatic remodeling of the transcriptome that strongly resembled that observed following SARS-CoV-2 infection. Moreover, Nsp14 expression altered the splicing of more than 1000 genes and resulted in a dramatic increase in the number of circRNAs, which are linked to innate immunity. These effects were independent of the Nsp14 exonuclease activity and required the N7-guanine-methyltransferase domain of the protein. Activation of the NFkB pathway and increased expression of CXCL8 occurred early upon Nsp14 expression. We identified IMPDH2, which catalyzes the rate-limiting step of guanine nucleotides biosynthesis, as a key mediator of these effects. Nsp14 expression caused an increase in GTP cellular levels, and the effect of Nsp14 was strongly decreased in the presence of IMPDH2 inhibitors. Together, our data demonstrate an unknown role for Nsp14 with implications for therapy. Twit Text FOAVip SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=35293857 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35293857 #FOAvip English Wikipedia <ref>{{Cite pmid|35293857}}</ref> SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome #MMPMID35293857 Zaffagni M; Harris JM; Patop IL; Pamudurti NR; Nguyen S; Kadener S Elife 2022[Mar]; 11 (ä): ä PMID35293857show ga Viral infection involves complex set of events orchestrated by multiple viral proteins. To identify functions of SARS-CoV-2 proteins, we performed transcriptomic analyses of cells expressing individual viral proteins. Expression of Nsp14, a protein involved in viral RNA replication, provoked a dramatic remodeling of the transcriptome that strongly resembled that observed following SARS-CoV-2 infection. Moreover, Nsp14 expression altered the splicing of more than 1000 genes and resulted in a dramatic increase in the number of circRNAs, which are linked to innate immunity. These effects were independent of the Nsp14 exonuclease activity and required the N7-guanine-methyltransferase domain of the protein. Activation of the NFkB pathway and increased expression of CXCL8 occurred early upon Nsp14 expression. We identified IMPDH2, which catalyzes the rate-limiting step of guanine nucleotides biosynthesis, as a key mediator of these effects. Nsp14 expression caused an increase in GTP cellular levels, and the effect of Nsp14 was strongly decreased in the presence of IMPDH2 inhibitors. Together, our data demonstrate an unknown role for Nsp14 with implications for therapy. |*COVID-19/genetics[MESH] |*SARS-CoV-2[MESH] |Exoribonucleases/metabolism[MESH] |Humans[MESH] |RNA, Viral/metabolism[MESH] |Transcriptome[MESH] |Viral Nonstructural Proteins/genetics/metabolism[MESH]SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host c(epmc).pdf DeepDyve Pubget Overpricing