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10.3390/ijms23052796 http://scihub22266oqcxt.onion/10.3390/ijms23052796 35269938!8911046!35269938     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\35269938.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Int+J+Mol+Sci 2022 ; 23 (5): ä Nephropedia Template TP {{tp|p=35269938|t=2022. Identification of Kukoamine A, Zeaxanthin, and Clexane as New Furin Inhibitors |pdf=|usr=}}{{35269938}} gab.com Text Identification of Kukoamine A, Zeaxanthin, and Clexane as New Furin Inhibitors JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=35269938 #FOAvip The endogenous protease furin is a key protein in many different diseases, such as cancer and infections. For this reason, a wide range of studies has focused on targeting furin from a therapeutic point of view. Our main objective consisted of identifying new compounds that could enlarge the furin inhibitor arsenal; secondarily, we assayed their adjuvant effect in combination with a known furin inhibitor, CMK, which avoids the SARS-CoV-2 S protein cleavage by means of that inhibition. Virtual screening was carried out to identify potential furin inhibitors. The inhibition of physiological and purified recombinant furin by screening selected compounds, Clexane, and these drugs in combination with CMK was assayed in fluorogenic tests by using a specific furin substrate. The effects of the selected inhibitors from virtual screening on cell viability (293T HEK cell line) were assayed by means of flow cytometry. Through virtual screening, Zeaxanthin and Kukoamine A were selected as the main potential furin inhibitors. In fluorogenic assays, these two compounds and Clexane inhibited both physiological and recombinant furin in a dose-dependent way. In addition, these compounds increased physiological furin inhibition by CMK, showing an adjuvant effect. In conclusion, we identified Kukoamine A, Zeaxanthin, and Clexane as new furin inhibitors. In addition, these drugs were able to increase furin inhibition by CMK, so they could also increase its efficiency when avoiding S protein proteolysis, which is essential for SARS-CoV-2 cell infection. Twit Text FOAVip Identification of Kukoamine A, Zeaxanthin, and Clexane as New Furin Inhibitors ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=35269938 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35269938 #FOAvip English Wikipedia <ref>{{Cite pmid|35269938}}</ref> Identification of Kukoamine A, Zeaxanthin, and Clexane as New Furin Inhibitors #MMPMID35269938 Zaragoza-Huesca D; Martinez-Cortes C; Banegas-Luna AJ; Perez-Garrido A; Vegara-Meseguer JM; Penas-Martinez J; Rodenas MC; Espin S; Perez-Sanchez H; Martinez-Martinez I Int J Mol Sci 2022[Mar]; 23 (5): ä PMID35269938show ga The endogenous protease furin is a key protein in many different diseases, such as cancer and infections. For this reason, a wide range of studies has focused on targeting furin from a therapeutic point of view. Our main objective consisted of identifying new compounds that could enlarge the furin inhibitor arsenal; secondarily, we assayed their adjuvant effect in combination with a known furin inhibitor, CMK, which avoids the SARS-CoV-2 S protein cleavage by means of that inhibition. Virtual screening was carried out to identify potential furin inhibitors. The inhibition of physiological and purified recombinant furin by screening selected compounds, Clexane, and these drugs in combination with CMK was assayed in fluorogenic tests by using a specific furin substrate. The effects of the selected inhibitors from virtual screening on cell viability (293T HEK cell line) were assayed by means of flow cytometry. Through virtual screening, Zeaxanthin and Kukoamine A were selected as the main potential furin inhibitors. In fluorogenic assays, these two compounds and Clexane inhibited both physiological and recombinant furin in a dose-dependent way. In addition, these compounds increased physiological furin inhibition by CMK, showing an adjuvant effect. In conclusion, we identified Kukoamine A, Zeaxanthin, and Clexane as new furin inhibitors. In addition, these drugs were able to increase furin inhibition by CMK, so they could also increase its efficiency when avoiding S protein proteolysis, which is essential for SARS-CoV-2 cell infection. |Amino Acid Chloromethyl Ketones/chemistry/metabolism/*pharmacology[MESH] |COVID-19/transmission/virology[MESH] |Catalytic Domain[MESH] |Cell Line, Tumor[MESH] |Cell Survival/drug effects[MESH] |Enoxaparin/chemistry/metabolism/*pharmacology[MESH] |Furin/*antagonists & inhibitors/chemistry/metabolism[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Molecular Docking Simulation[MESH] |Molecular Structure[MESH] |Protease Inhibitors/chemistry/metabolism/pharmacology[MESH] |Proteolysis[MESH] |SARS-CoV-2/metabolism/physiology[MESH] |Spermine/*analogs & derivatives/chemistry/metabolism/pharmacology[MESH] |Spike Glycoprotein, Coronavirus/metabolism[MESH] |Virus Internalization[MESH] |Virus Replication[MESH]Identification of Kukoamine A, Zeaxanthin, and Clexane as New Furin In(epmc).pdf DeepDyve Pubget Overpricing