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10.1038/s41598-022-07867-0 http://scihub22266oqcxt.onion/10.1038/s41598-022-07867-0 35260703!8904465!35260703     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2022 ; 12 (1): 3794 Nephropedia Template TP {{tp|p=35260703|t=2022. An intra-cytoplasmic route for SARS-CoV-2 transmission unveiled by Helium-ion microscopy |pdf=|usr=}}{{35260703}} gab.com Text An intra-cytoplasmic route for SARS-CoV-2 transmission unveiled by Helium-ion microscopy JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=35260703 #FOAvip SARS-CoV-2 virions enter the host cells by docking their spike glycoproteins to the membrane-bound Angiotensin Converting Enzyme 2. After intracellular assembly, the newly formed virions are released from the infected cells to propagate the infection, using the extra-cytoplasmic ACE2 docking mechanism. However, the molecular events underpinning SARS-CoV-2 transmission between host cells are not fully understood. Here, we report the findings of a scanning Helium-ion microscopy study performed on Vero E6 cells infected with mNeonGreen-expressing SARS-CoV-2. Our data reveal, with unprecedented resolution, the presence of: (1) long tunneling nanotubes that connect two or more host cells over submillimeter distances; (2) large scale multiple cell fusion events (syncytia); and (3) abundant extracellular vesicles of various sizes. Taken together, these ultrastructural features describe a novel intra-cytoplasmic connection among SARS-CoV-2 infected cells that may act as an alternative route of viral transmission, disengaged from the well-known extra-cytoplasmic ACE2 docking mechanism. Such route may explain the elusiveness of SARS-CoV-2 to survive from the immune surveillance of the infected host. Twit Text FOAVip An intra-cytoplasmic route for SARS-CoV-2 transmission unveiled by Helium-ion microscopy ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=35260703 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35260703 #FOAvip English Wikipedia <ref>{{Cite pmid|35260703}}</ref> An intra-cytoplasmic route for SARS-CoV-2 transmission unveiled by Helium-ion microscopy #MMPMID35260703 Merolli A; Kasaei L; Ramasamy S; Kolloli A; Kumar R; Subbian S; Feldman LC Sci Rep 2022[Mar]; 12 (1): 3794 PMID35260703show ga SARS-CoV-2 virions enter the host cells by docking their spike glycoproteins to the membrane-bound Angiotensin Converting Enzyme 2. After intracellular assembly, the newly formed virions are released from the infected cells to propagate the infection, using the extra-cytoplasmic ACE2 docking mechanism. However, the molecular events underpinning SARS-CoV-2 transmission between host cells are not fully understood. Here, we report the findings of a scanning Helium-ion microscopy study performed on Vero E6 cells infected with mNeonGreen-expressing SARS-CoV-2. Our data reveal, with unprecedented resolution, the presence of: (1) long tunneling nanotubes that connect two or more host cells over submillimeter distances; (2) large scale multiple cell fusion events (syncytia); and (3) abundant extracellular vesicles of various sizes. Taken together, these ultrastructural features describe a novel intra-cytoplasmic connection among SARS-CoV-2 infected cells that may act as an alternative route of viral transmission, disengaged from the well-known extra-cytoplasmic ACE2 docking mechanism. Such route may explain the elusiveness of SARS-CoV-2 to survive from the immune surveillance of the infected host. |*Virus Internalization[MESH] |Angiotensin-Converting Enzyme 2/metabolism[MESH] |Animals[MESH] |COVID-19/transmission/virology[MESH] |Chlorocebus aethiops[MESH] |Cytoplasm/chemistry/ultrastructure/virology[MESH] |Extracellular Vesicles/chemistry/ultrastructure[MESH] |Giant Cells/chemistry/physiology[MESH] |Helium/chemistry[MESH] |Humans[MESH] |Ions/chemistry[MESH] |Microscopy/*methods[MESH] |SARS-CoV-2/isolation & purification/*physiology[MESH] |Spike Glycoprotein, Coronavirus/chemistry/metabolism[MESH]An intra-cytoplasmic route for SARS-CoV-2 transmission unveiled by Hel(epmc).pdf DeepDyve Pubget Overpricing