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10.1093/nar/gkac144 http://scihub22266oqcxt.onion/10.1093/nar/gkac144 35244721!8989550!35244721     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2022 ; 50 (6): 3475-3489 Nephropedia Template TP {{tp|p=35244721|t=2022. Nanopore ReCappable sequencing maps SARS-CoV-2 5 capping sites and provides new insights into the structure of sgRNAs |pdf=|usr=}}{{35244721}} gab.com Text Nanopore ReCappable sequencing maps SARS-CoV-2 5 capping sites and provides new insights into the structure of sgRNAs JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=35244721 #FOAvip The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA sequencing can recover full-length transcripts, greatly simplifying the assembly of structurally complex RNAs. However, these techniques do not detect the 5' cap, thus preventing reliable identification and quantification of full-length, coding transcript models. Here we used Nanopore ReCappable Sequencing (NRCeq), a new technique that can identify capped full-length RNAs, to assemble a complete annotation of SARS-CoV-2 sgRNAs and annotate the location of capping sites across the viral genome. We obtained robust estimates of sgRNA expression across cell lines and viral isolates and identified novel canonical and non-canonical sgRNAs, including one that uses a previously un-annotated leader-to-body junction site. The data generated in this work constitute a useful resource for the scientific community and provide important insights into the mechanisms that regulate the transcription of SARS-CoV-2 sgRNAs. Twit Text FOAVip Nanopore ReCappable sequencing maps SARS-CoV-2 5 capping sites and provides new insights into the structure of sgRNAs ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=35244721 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35244721 #FOAvip English Wikipedia <ref>{{Cite pmid|35244721}}</ref> Nanopore ReCappable sequencing maps SARS-CoV-2 5 capping sites and provides new insights into the structure of sgRNAs #MMPMID35244721 Ugolini C; Mulroney L; Leger A; Castelli M; Criscuolo E; Williamson MK; Davidson AD; Almuqrin A; Giambruno R; Jain M; Frige G; Olsen H; Tzertzinis G; Schildkraut I; Wulf MG; Correa IR; Ettwiller L; Clementi N; Clementi M; Mancini N; Birney E; Akeson M; Nicassio F; Matthews DA; Leonardi T Nucleic Acids Res 2022[Apr]; 50 (6): 3475-3489 PMID35244721show ga The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA sequencing can recover full-length transcripts, greatly simplifying the assembly of structurally complex RNAs. However, these techniques do not detect the 5' cap, thus preventing reliable identification and quantification of full-length, coding transcript models. Here we used Nanopore ReCappable Sequencing (NRCeq), a new technique that can identify capped full-length RNAs, to assemble a complete annotation of SARS-CoV-2 sgRNAs and annotate the location of capping sites across the viral genome. We obtained robust estimates of sgRNA expression across cell lines and viral isolates and identified novel canonical and non-canonical sgRNAs, including one that uses a previously un-annotated leader-to-body junction site. The data generated in this work constitute a useful resource for the scientific community and provide important insights into the mechanisms that regulate the transcription of SARS-CoV-2 sgRNAs. |*COVID-19/genetics[MESH] |*Nanopores[MESH] |Genome, Viral/genetics[MESH] |Humans[MESH] |RNA Caps[MESH] |RNA, Viral/genetics/metabolism[MESH]Nanopore ReCappable sequencing maps SARS-CoV-2 5 capping sites and pr(epmc).pdf DeepDyve Pubget Overpricing