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10.1038/s41467-022-28603-2 http://scihub22266oqcxt.onion/10.1038/s41467-022-28603-2 35236847!8891300!35236847     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2022 ; 13 (1): 1128 Nephropedia Template TP {{tp|p=35236847|t=2022. Secondary structural ensembles of the SARS-CoV-2 RNA genome in infected cells |pdf=|usr=}}{{35236847}} gab.com Text Secondary structural ensembles of the SARS-CoV-2 RNA genome in infected cells JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=35236847 #FOAvip SARS-CoV-2 is a betacoronavirus with a single-stranded, positive-sense, 30-kilobase RNA genome responsible for the ongoing COVID-19 pandemic. Although population average structure models of the genome were recently reported, there is little experimental data on native structural ensembles, and most structures lack functional characterization. Here we report secondary structure heterogeneity of the entire SARS-CoV-2 genome in two lines of infected cells at single nucleotide resolution. Our results reveal alternative RNA conformations across the genome and at the critical frameshifting stimulation element (FSE) that are drastically different from prevailing population average models. Importantly, we find that this structural ensemble promotes frameshifting rates much higher than the canonical minimal FSE and similar to ribosome profiling studies. Our results highlight the value of studying RNA in its full length and cellular context. The genomic structures detailed here lay groundwork for coronavirus RNA biology and will guide the design of SARS-CoV-2 RNA-based therapeutics. Twit Text FOAVip Secondary structural ensembles of the SARS-CoV-2 RNA genome in infected cells ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=35236847 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35236847 #FOAvip English Wikipedia <ref>{{Cite pmid|35236847}}</ref> Secondary structural ensembles of the SARS-CoV-2 RNA genome in infected cells #MMPMID35236847 Lan TCT; Allan MF; Malsick LE; Woo JZ; Zhu C; Zhang F; Khandwala S; Nyeo SSY; Sun Y; Guo JU; Bathe M; Naar A; Griffiths A; Rouskin S Nat Commun 2022[Mar]; 13 (1): 1128 PMID35236847show ga SARS-CoV-2 is a betacoronavirus with a single-stranded, positive-sense, 30-kilobase RNA genome responsible for the ongoing COVID-19 pandemic. Although population average structure models of the genome were recently reported, there is little experimental data on native structural ensembles, and most structures lack functional characterization. Here we report secondary structure heterogeneity of the entire SARS-CoV-2 genome in two lines of infected cells at single nucleotide resolution. Our results reveal alternative RNA conformations across the genome and at the critical frameshifting stimulation element (FSE) that are drastically different from prevailing population average models. Importantly, we find that this structural ensemble promotes frameshifting rates much higher than the canonical minimal FSE and similar to ribosome profiling studies. Our results highlight the value of studying RNA in its full length and cellular context. The genomic structures detailed here lay groundwork for coronavirus RNA biology and will guide the design of SARS-CoV-2 RNA-based therapeutics. |COVID-19/*virology[MESH] |Frameshifting, Ribosomal[MESH] |Genome, Viral[MESH] |Humans[MESH] |Nucleic Acid Conformation[MESH] |RNA, Viral/*chemistry/genetics/metabolism[MESH]Secondary structural ensembles of the SARS-CoV-2 RNA genome in infecte(epmc).pdf DeepDyve Pubget Overpricing