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10.1101/2022.02.21.481223 http://scihub22266oqcxt.onion/10.1101/2022.02.21.481223 35233578!8887137!35233578     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=35233578&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       bioRxiv 2022 ; ä (ä): ä Nephropedia Template TP {{tp|p=35233578|t=2022. Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions |pdf=|usr=}}{{35233578}} gab.com Text Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions JO: bioRxiv http://www.kidney.de/pget.php?&tw=1&pmid=35233578 #FOAvip The COVID-19 pandemic is caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The betacoronvirus has a positive sense RNA genome which encodes for several RNA binding proteins. Here, we use enhanced crosslinking and immunoprecipitation to investigate SARS-CoV-2 protein interactions with viral and host RNAs in authentic virus-infected cells. SARS-CoV-2 proteins, NSP8, NSP12, and nucleocapsid display distinct preferences to specific regions in the RNA viral genome, providing evidence for their shared and separate roles in replication, transcription, and viral packaging. SARS-CoV-2 proteins expressed in human lung epithelial cells bind to 4773 unique host coding RNAs. Nine SARS-CoV-2 proteins upregulate target gene expression, including NSP12 and ORF9c, whose RNA substrates are associated with pathways in protein N-linked glycosylation ER processing and mitochondrial processes. Furthermore, siRNA knockdown of host genes targeted by viral proteins in human lung organoid cells identify potential antiviral host targets across different SARS-CoV-2 variants. Conversely, NSP9 inhibits host gene expression by blocking mRNA export and dampens cytokine productions, including interleukin-1alpha/beta. Our viral protein-RNA interactome provides a catalog of potential therapeutic targets and offers insight into the etiology of COVID-19 as a safeguard against future pandemics. Twit Text FOAVip Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions ????bioRxiv http://www.kidney.de/pget.php?&tw=1&pmid=35233578 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35233578 #FOAvip English Wikipedia <ref>{{Cite pmid|35233578}}</ref> Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions #MMPMID35233578 Xiang JS; Mueller JR; Luo EC; Yee BA; Schafer D; Schmok JC; Tan FE; Rothamel K; McVicar RN; Kwong EM; Jones KL; Her HL; Chen CY; Vu AQ; Jin W; Park SS; Le P; Brannan KW; Kofman ER; Li Y; Tankka AT; Dong KD; Song Y; Carlin AF; Van Nostrand EL; Leibel SL; Yeo GW bioRxiv 2022[Feb]; ä (ä): ä PMID35233578show ga The COVID-19 pandemic is caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The betacoronvirus has a positive sense RNA genome which encodes for several RNA binding proteins. Here, we use enhanced crosslinking and immunoprecipitation to investigate SARS-CoV-2 protein interactions with viral and host RNAs in authentic virus-infected cells. SARS-CoV-2 proteins, NSP8, NSP12, and nucleocapsid display distinct preferences to specific regions in the RNA viral genome, providing evidence for their shared and separate roles in replication, transcription, and viral packaging. SARS-CoV-2 proteins expressed in human lung epithelial cells bind to 4773 unique host coding RNAs. Nine SARS-CoV-2 proteins upregulate target gene expression, including NSP12 and ORF9c, whose RNA substrates are associated with pathways in protein N-linked glycosylation ER processing and mitochondrial processes. Furthermore, siRNA knockdown of host genes targeted by viral proteins in human lung organoid cells identify potential antiviral host targets across different SARS-CoV-2 variants. Conversely, NSP9 inhibits host gene expression by blocking mRNA export and dampens cytokine productions, including interleukin-1alpha/beta. Our viral protein-RNA interactome provides a catalog of potential therapeutic targets and offers insight into the etiology of COVID-19 as a safeguard against future pandemics. äDiscovery and functional interrogation of SARS-CoV-2 protein-RNA inter(epmc).pdf DeepDyve Pubget Overpricing