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Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 STAR+Protoc 2022 ; 3 (1): 101177 Nephropedia Template TP
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Mass spectrometry and proteome analysis to identify SARS-CoV-2 protein from COVID-19 patient swab samples #MMPMID35233542
Banerjee A; Pai MGJ; Singh A; Nissa MU; Srivastava S
STAR Protoc 2022[Mar]; 3 (1): 101177 PMID35233542show ga
With new emerging SARS-CoV-2 strains and their increased pathogenicity, diagnosis has become more challenging. Molecular diagnosis often involves the use of nasopharyngeal swabs and subsequent real-time PCR-based tests. Although this test is the gold standard, it has several limitations; therefore, more complementary assays are required. This protocol describes how to identify SARS-CoV-2 protein from patients' nasopharyngeal swab samples. We first introduce the approach of label-free quantitative proteomics. We then detail target verification by triple quadrupole mass spectrometry (MS)-based targeted proteomics. For complete details on the use and execution of this profile, please refer to Bankar et al. (2021).