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10.1016/j.xpro.2022.101177

http://scihub22266oqcxt.onion/10.1016/j.xpro.2022.101177
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35233542!8808698!35233542
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suck abstract from ncbi


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pmid35233542      STAR+Protoc 2022 ; 3 (1): 101177
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  • Mass spectrometry and proteome analysis to identify SARS-CoV-2 protein from COVID-19 patient swab samples #MMPMID35233542
  • Banerjee A; Pai MGJ; Singh A; Nissa MU; Srivastava S
  • STAR Protoc 2022[Mar]; 3 (1): 101177 PMID35233542show ga
  • With new emerging SARS-CoV-2 strains and their increased pathogenicity, diagnosis has become more challenging. Molecular diagnosis often involves the use of nasopharyngeal swabs and subsequent real-time PCR-based tests. Although this test is the gold standard, it has several limitations; therefore, more complementary assays are required. This protocol describes how to identify SARS-CoV-2 protein from patients' nasopharyngeal swab samples. We first introduce the approach of label-free quantitative proteomics. We then detail target verification by triple quadrupole mass spectrometry (MS)-based targeted proteomics. For complete details on the use and execution of this profile, please refer to Bankar et al. (2021).
  • |*Proteomics[MESH]
  • |*Specimen Handling[MESH]
  • |*Tandem Mass Spectrometry[MESH]
  • |COVID-19/*metabolism[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Nasopharynx/*metabolism/virology[MESH]
  • |SARS-CoV-2/*metabolism[MESH]


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