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10.31083/j.fbl2702048 http://scihub22266oqcxt.onion/10.31083/j.fbl2702048 35226991!ä!35226991 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Biosci+(Landmark+Ed) 2022 ; 27 (2): 48 Nephropedia Template TP {{tp|p=35226991|t=2022. Thymosin-alpha1 binds with ACE and downregulates the expression of ACE2 in human respiratory epithelia |pdf=|usr=}}{{35226991}} gab.com Text Thymosin-alpha1 binds with ACE and downregulates the expression of ACE2 in human respiratory epithelia JO: Front Biosci (Landmark Ed) http://www.kidney.de/pget.php?&tw=1&pmid=35226991 #FOAvip BACKGROUND: Thymosin-alpha1 has been implicated into the treatment of novel respiratory virus Coronavirus Disease 2019 (COVID-19), but the underlying mechanisms are still disputable. AIM: Herein we aimed to reveal a previously unrecognized mechanism that thymosin-alpha1 prevents COVID-19 by binding with angiotensin-converting enzyme (ACE), which was inspired from the tool of network pharmacology. METHODS: KEGG pathway enrichment of thymosin-alpha1 treating COVID-19 was analyzed by Database of Functional Annotation Bioinformatics Microarray Analysis, then core targets were validated by ligand binding kinetics assay and fluorometric detection of ACE and ACE2 enzymatic activity. The production of angiotensin I, angiotensin II, angiotensin (1-7) and angiotensin (1-9) were detected by enzyme linked immunosorbent assay. RESULTS: We found that thymosin-alpha1 impaired the expressions of angiotensin-converting enzyme 2 and angiotensin (1-7) of human lung epithelial cells in a dose-dependent way (p < 0.001). In contrast, thymosin-alpha1 had no impact on their ACE and angiotensin (1-9) expressions but significantly inhibited the enzymatic activity of ACE (p > 0.05). CONCLUSION: The bioinformatic findings of network pharmacology and the corresponding pharmacological validations have revealed that thymosin-alpha1 treatment could decrease ACE2 expression in human lung epithelial cells, which strengthens the potential clinical applications of thymosin-alpha1 to prevent severe acute respiratory syndrome coronavirus 2 infection. Twit Text FOAVip Thymosin-alpha1 binds with ACE and downregulates the expression of ACE2 in human respiratory epithelia ????Front Biosci (Landmark Ed) http://www.kidney.de/pget.php?&tw=1&pmid=35226991 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35226991 #FOAvip English Wikipedia <ref>{{Cite pmid|35226991}}</ref> Thymosin-alpha1 binds with ACE and downregulates the expression of ACE2 in human respiratory epithelia #MMPMID35226991 Zhang YH; Wang WY; Pang XC; Wang Z; Wang CZ; Zhou H; Zheng B; Cui YM Front Biosci (Landmark Ed) 2022[Feb]; 27 (2): 48 PMID35226991show ga BACKGROUND: Thymosin-alpha1 has been implicated into the treatment of novel respiratory virus Coronavirus Disease 2019 (COVID-19), but the underlying mechanisms are still disputable. AIM: Herein we aimed to reveal a previously unrecognized mechanism that thymosin-alpha1 prevents COVID-19 by binding with angiotensin-converting enzyme (ACE), which was inspired from the tool of network pharmacology. METHODS: KEGG pathway enrichment of thymosin-alpha1 treating COVID-19 was analyzed by Database of Functional Annotation Bioinformatics Microarray Analysis, then core targets were validated by ligand binding kinetics assay and fluorometric detection of ACE and ACE2 enzymatic activity. The production of angiotensin I, angiotensin II, angiotensin (1-7) and angiotensin (1-9) were detected by enzyme linked immunosorbent assay. RESULTS: We found that thymosin-alpha1 impaired the expressions of angiotensin-converting enzyme 2 and angiotensin (1-7) of human lung epithelial cells in a dose-dependent way (p < 0.001). In contrast, thymosin-alpha1 had no impact on their ACE and angiotensin (1-9) expressions but significantly inhibited the enzymatic activity of ACE (p > 0.05). CONCLUSION: The bioinformatic findings of network pharmacology and the corresponding pharmacological validations have revealed that thymosin-alpha1 treatment could decrease ACE2 expression in human lung epithelial cells, which strengthens the potential clinical applications of thymosin-alpha1 to prevent severe acute respiratory syndrome coronavirus 2 infection. |*Angiotensin-Converting Enzyme 2[MESH] |*COVID-19 Drug Treatment[MESH] |Angiotensin-Converting Enzyme Inhibitors/pharmacology/therapeutic use[MESH] |Humans[MESH] |SARS-CoV-2[MESH]Thymosin-alpha1 binds with ACE and downregulates the expression of ACE(epmc).pdf DeepDyve Pubget Overpricing