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  • Secondary Structure of Subgenomic RNA M of SARS-CoV-2 #MMPMID35215915
  • Soszynska-Jozwiak M; Ruszkowska A; Kierzek R; O'Leary CA; Moss WN; Kierzek E
  • Viruses 2022[Feb]; 14 (2): ä PMID35215915show ga
  • SARS-CoV-2 belongs to the Coronavirinae family. Like other coronaviruses, SARS-CoV-2 is enveloped and possesses a positive-sense, single-stranded RNA genome of ~30 kb. Genomic RNA is used as the template for replication and transcription. During these processes, positive-sense genomic RNA (gRNA) and subgenomic RNAs (sgRNAs) are created. Several studies presented the importance of the genomic RNA secondary structure in SARS-CoV-2 replication. However, the structure of sgRNAs has remained largely unsolved so far. In this study, we probed the sgRNA M model of SARS-CoV-2 in vitro. The presented model molecule includes 5'UTR and a coding sequence of gene M. This is the first experimentally informed secondary structure model of sgRNA M, which presents features likely to be important in sgRNA M function. The knowledge of sgRNA M structure provides insights to better understand virus biology and could be used for designing new therapeutics.
  • |*Genome, Viral[MESH]
  • |5' Untranslated Regions[MESH]
  • |COVID-19/virology[MESH]
  • |Genomics[MESH]
  • |Humans[MESH]
  • |Open Reading Frames[MESH]
  • |RNA, Viral/*chemistry/genetics[MESH]
  • |SARS-CoV-2/*genetics[MESH]
  • |Transcription, Genetic[MESH]

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  • suck abstract from ncbi

    ä 2.14 2022