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10.3390/v14020201 http://scihub22266oqcxt.onion/10.3390/v14020201 35215794!8879486!35215794     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2022 ; 14 (2): ä Nephropedia Template TP {{tp|p=35215794|t=2022. SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System |pdf=|usr=}}{{35215794}} gab.com Text SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=35215794 #FOAvip The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted public health and the world economy and fueled a worldwide race to approve therapeutic and prophylactic agents, but so far there are no specific antiviral drugs. Understanding the biology of the virus is the first step in structuring strategies to combat it, and in this context several studies have been conducted with the aim of understanding the replication mechanism of SARS-CoV-2 in vitro systems. In this work, studies using transmission and scanning electron microscopy and 3D electron microscopy modeling were performed with the goal of characterizing the morphogenesis of SARS-CoV-2 in Vero-E6 cells. Several ultrastructural changes were observed-such as syncytia formation, cytoplasmic membrane projections, lipid droplets accumulation, proliferation of double-membrane vesicles derived from the rough endoplasmic reticulum, and alteration of mitochondria. The entry of the virus into cells occurred through endocytosis. Viral particles were observed attached to the cell membrane and in various cellular compartments, and extrusion of viral progeny took place by exocytosis. These findings allow us to infer that Vero-E6 cells are highly susceptible to SARS-CoV-2 infection as described in the literature and their replication cycle is similar to that described with SARS-CoV and MERS-CoV in vitro models. Twit Text FOAVip SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=35215794 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35215794 #FOAvip English Wikipedia <ref>{{Cite pmid|35215794}}</ref> SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System #MMPMID35215794 Barreto-Vieira DF; da Silva MAN; de Almeida ALT; Rasinhas ADC; Monteiro ME; Miranda MD; Motta FC; Siqueira MM; Girard-Dias W; Archanjo BS; Bozza PT; L Souza TM; Gomes Dias SS; Soares VC; Barth OM Viruses 2022[Jan]; 14 (2): ä PMID35215794show ga The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted public health and the world economy and fueled a worldwide race to approve therapeutic and prophylactic agents, but so far there are no specific antiviral drugs. Understanding the biology of the virus is the first step in structuring strategies to combat it, and in this context several studies have been conducted with the aim of understanding the replication mechanism of SARS-CoV-2 in vitro systems. In this work, studies using transmission and scanning electron microscopy and 3D electron microscopy modeling were performed with the goal of characterizing the morphogenesis of SARS-CoV-2 in Vero-E6 cells. Several ultrastructural changes were observed-such as syncytia formation, cytoplasmic membrane projections, lipid droplets accumulation, proliferation of double-membrane vesicles derived from the rough endoplasmic reticulum, and alteration of mitochondria. The entry of the virus into cells occurred through endocytosis. Viral particles were observed attached to the cell membrane and in various cellular compartments, and extrusion of viral progeny took place by exocytosis. These findings allow us to infer that Vero-E6 cells are highly susceptible to SARS-CoV-2 infection as described in the literature and their replication cycle is similar to that described with SARS-CoV and MERS-CoV in vitro models. |Animals[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Microscopy, Electron, Transmission/*methods[MESH] |Microscopy, Electron/*methods[MESH] |SARS-CoV-2/chemistry/*metabolism/*ultrastructure[MESH] |Vero Cells[MESH] |Virus Internalization[MESH]SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In(epmc).pdf DeepDyve Pubget Overpricing