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10.3390/genes13020348

http://scihub22266oqcxt.onion/10.3390/genes13020348
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35205390!8871708!35205390
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suck abstract from ncbi


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pmid35205390      Genes+(Basel) 2022 ; 13 (2): ä
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  • Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2 #MMPMID35205390
  • Eichmeier A; Kiss T; Kocanova M; Hakalova E; Spetik M; Cechova J; Tichy B
  • Genes (Basel) 2022[Feb]; 13 (2): ä PMID35205390show ga
  • The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles. We explored differentially expressed miRNAs in response to SARS-CoV-2 in the RNA extracted from nasopharynx tissues of 10 SARS-CoV-2-positive and 10 SARS-CoV-2-negative patients. miRNAs were identified by small RNA sequencing, and the expression levels of selected miRNAs were validated by real-time RT-PCR. We identified 943 conserved miRNAs, likely generated through posttranscriptional modifications. The identified miRNAs were expressed in both RNA groups, NegS and PosS: miR-148a, miR-21, miR-34c, miR-34b, and miR-342. The most differentially expressed miRNA was miR-21, which is likely closely linked to the presence of SARS-CoV-2 in nasopharynx tissues. Our results contribute to further understanding the role of miRNAs in SARS-CoV-2 pathogenesis, which may be crucial for understanding disease symptom development in humans.
  • |COVID-19/pathology/virology[MESH]
  • |Down-Regulation[MESH]
  • |High-Throughput Nucleotide Sequencing[MESH]
  • |Humans[MESH]
  • |MicroRNAs/chemistry/*metabolism[MESH]
  • |Nasopharynx/*metabolism/virology[MESH]
  • |Principal Component Analysis[MESH]
  • |RNA, Viral/metabolism[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2/genetics/isolation & purification/*physiology[MESH]
  • |Sequence Analysis, RNA[MESH]
  • |Transcriptome[MESH]


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