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10.1016/j.xpro.2022.101156 http://scihub22266oqcxt.onion/10.1016/j.xpro.2022.101156 35141569!8769939!35141569     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2022 ; 3 (1): 101156 Nephropedia Template TP {{tp|p=35141569|t=2022. Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues from COVID-19 patients |pdf=|usr=}}{{35141569}} gab.com Text Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues from COVID-19 patients JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=35141569 #FOAvip Here we present an optimized protocol for transcriptome profiling of COVID-19 patient samples, including peripheral blood mononuclear cells (PBMCs) and formalin-fixed paraffin-embedded tissue samples obtained from the lung, liver, heart, kidney, and spleen, with the matched controls. We describe RNA extraction and subsequent transcriptome analysis using NanoString technology of the patient samples. The protocol provides information about sample preparation, RNA extraction, and NanoString profiling and analysis. It can be also applied to differentiated Th17 and Treg subsets or formalin-fixed colon tissue samples. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021). Twit Text FOAVip Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues from COVID-19 patients ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=35141569 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35141569 #FOAvip English Wikipedia <ref>{{Cite pmid|35141569}}</ref> Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues from COVID-19 patients #MMPMID35141569 Vuerich M; Wang N; Kalbasi A; Graham JJ; Longhi MS STAR Protoc 2022[Mar]; 3 (1): 101156 PMID35141569show ga Here we present an optimized protocol for transcriptome profiling of COVID-19 patient samples, including peripheral blood mononuclear cells (PBMCs) and formalin-fixed paraffin-embedded tissue samples obtained from the lung, liver, heart, kidney, and spleen, with the matched controls. We describe RNA extraction and subsequent transcriptome analysis using NanoString technology of the patient samples. The protocol provides information about sample preparation, RNA extraction, and NanoString profiling and analysis. It can be also applied to differentiated Th17 and Treg subsets or formalin-fixed colon tissue samples. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021). |*Transcriptome[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Autopsy[MESH] |COVID-19/*diagnosis/genetics/virology[MESH] |Case-Control Studies[MESH] |Female[MESH] |Formaldehyde/*chemistry[MESH] |Humans[MESH] |Leukocytes, Mononuclear/*metabolism[MESH] |Male[MESH] |Middle Aged[MESH] |RNA, Viral/analysis/*genetics[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Tissue Fixation/*methods[MESH]Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues fr(epmc).pdf DeepDyve Pubget Overpricing