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  • Sequencing SARS-CoV-2 from antigen tests #MMPMID35134077
  • Nazario-Toole A; Nguyen HM; Xia H; Frankel DN; Kieffer JW; Gibbons TF
  • PLoS One 2022[]; 17 (2): e0263794 PMID35134077show ga
  • Genomic surveillance empowers agile responses to SARS-CoV-2 by enabling scientists and public health analysts to issue recommendations aimed at slowing transmission, prioritizing contact tracing, and building a robust genomic sequencing surveillance strategy. Since the start of the pandemic, real time RT-PCR diagnostic testing from upper respiratory specimens, such as nasopharyngeal (NP) swabs, has been the standard. Moreover, respiratory samples in viral transport media are the ideal specimen for SARS-CoV-2 whole-genome sequencing (WGS). In early 2021, many clinicians transitioned to antigen-based SARS-CoV-2 detection tests, which use anterior nasal swabs for SARS-CoV-2 antigen detection. Despite this shift in testing methods, the need for whole-genome sequence surveillance remains. Thus, we developed a workflow for whole-genome sequencing with antigen test-derived swabs as an input rather than nasopharyngeal swabs. In this study, we use excess clinical specimens processed using the BinaxNOW COVID-19 Ag Card. We demonstrate that whole-genome sequencing from antigen tests is feasible and yields similar results from RT-PCR-based assays utilizing a swab in viral transport media.
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19/*diagnosis/genetics/virology[MESH]
  • |Culture Media/*analysis/metabolism[MESH]
  • |High-Throughput Nucleotide Sequencing/*methods[MESH]
  • |Humans[MESH]
  • |SARS-CoV-2/*genetics/isolation & purification[MESH]
  • |Specimen Handling/*methods[MESH]
  • |Whole Genome Sequencing/*methods[MESH]

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  • suck abstract from ncbi

    e0263794 2.17 2022