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10.1371/journal.pone.0263794 http://scihub22266oqcxt.onion/10.1371/journal.pone.0263794 35134077!8824375!35134077     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=35134077&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\35134077.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2022 ; 17 (2): e0263794 Nephropedia Template TP {{tp|p=35134077|t=2022. Sequencing SARS-CoV-2 from antigen tests |pdf=|usr=}}{{35134077}} gab.com Text Sequencing SARS-CoV-2 from antigen tests JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=35134077 #FOAvip Genomic surveillance empowers agile responses to SARS-CoV-2 by enabling scientists and public health analysts to issue recommendations aimed at slowing transmission, prioritizing contact tracing, and building a robust genomic sequencing surveillance strategy. Since the start of the pandemic, real time RT-PCR diagnostic testing from upper respiratory specimens, such as nasopharyngeal (NP) swabs, has been the standard. Moreover, respiratory samples in viral transport media are the ideal specimen for SARS-CoV-2 whole-genome sequencing (WGS). In early 2021, many clinicians transitioned to antigen-based SARS-CoV-2 detection tests, which use anterior nasal swabs for SARS-CoV-2 antigen detection. Despite this shift in testing methods, the need for whole-genome sequence surveillance remains. Thus, we developed a workflow for whole-genome sequencing with antigen test-derived swabs as an input rather than nasopharyngeal swabs. In this study, we use excess clinical specimens processed using the BinaxNOW COVID-19 Ag Card. We demonstrate that whole-genome sequencing from antigen tests is feasible and yields similar results from RT-PCR-based assays utilizing a swab in viral transport media. Twit Text FOAVip Sequencing SARS-CoV-2 from antigen tests ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=35134077 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35134077 #FOAvip English Wikipedia <ref>{{Cite pmid|35134077}}</ref> Sequencing SARS-CoV-2 from antigen tests #MMPMID35134077 Nazario-Toole A; Nguyen HM; Xia H; Frankel DN; Kieffer JW; Gibbons TF PLoS One 2022[]; 17 (2): e0263794 PMID35134077show ga Genomic surveillance empowers agile responses to SARS-CoV-2 by enabling scientists and public health analysts to issue recommendations aimed at slowing transmission, prioritizing contact tracing, and building a robust genomic sequencing surveillance strategy. Since the start of the pandemic, real time RT-PCR diagnostic testing from upper respiratory specimens, such as nasopharyngeal (NP) swabs, has been the standard. Moreover, respiratory samples in viral transport media are the ideal specimen for SARS-CoV-2 whole-genome sequencing (WGS). In early 2021, many clinicians transitioned to antigen-based SARS-CoV-2 detection tests, which use anterior nasal swabs for SARS-CoV-2 antigen detection. Despite this shift in testing methods, the need for whole-genome sequence surveillance remains. Thus, we developed a workflow for whole-genome sequencing with antigen test-derived swabs as an input rather than nasopharyngeal swabs. In this study, we use excess clinical specimens processed using the BinaxNOW COVID-19 Ag Card. We demonstrate that whole-genome sequencing from antigen tests is feasible and yields similar results from RT-PCR-based assays utilizing a swab in viral transport media. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/genetics/virology[MESH] |Culture Media/*analysis/metabolism[MESH] |High-Throughput Nucleotide Sequencing/*methods[MESH] |Humans[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Specimen Handling/*methods[MESH]Sequencing SARS-CoV-2 from antigen tests (epmc).pdf DeepDyve Pubget Overpricing