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10.1016/j.crmeth.2022.100170 http://scihub22266oqcxt.onion/10.1016/j.crmeth.2022.100170 35128513!8806145!35128513     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=35128513&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Cell+Rep+Methods 2022 ; 2 (2): 100170 Nephropedia Template TP {{tp|p=35128513|t=2022. Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection |pdf=|usr=}}{{35128513}} gab.com Text Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection JO: Cell Rep Methods http://www.kidney.de/pget.php?&tw=1&pmid=35128513 #FOAvip The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Using the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multi-color RNA immunoFISH and visualized their localization patterns within the cell. The 10-nm resolution achieved by our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive imaging framework that will enable future investigations of coronavirus fundamental biology and therapeutic effects. Twit Text FOAVip Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection ????Cell Rep Methods http://www.kidney.de/pget.php?&tw=1&pmid=35128513 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35128513 #FOAvip English Wikipedia <ref>{{Cite pmid|35128513}}</ref> Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection #MMPMID35128513 Wang J; Han M; Roy AR; Wang H; Mockl L; Zeng L; Moerner WE; Qi LS Cell Rep Methods 2022[Feb]; 2 (2): 100170 PMID35128513show ga The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Using the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multi-color RNA immunoFISH and visualized their localization patterns within the cell. The 10-nm resolution achieved by our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive imaging framework that will enable future investigations of coronavirus fundamental biology and therapeutic effects. |*COVID-19[MESH] |*Coronavirus 229E, Human/genetics[MESH] |Cell Line[MESH] |Humans[MESH] |RNA, Double-Stranded/pharmacology[MESH]Multi-color super-resolution imaging to study human coronavirus RNA du(epmc).pdf DeepDyve Pubget Overpricing