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10.1016/j.snr.2020.100017

http://scihub22266oqcxt.onion/10.1016/j.snr.2020.100017
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35047828!7428436!35047828
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suck abstract from ncbi


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pmid35047828      Sens+Actuators+Rep 2020 ; 2 (1): 100017
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  • Mini review: Recent progress in RT-LAMP enabled COVID-19 detection #MMPMID35047828
  • Thompson D; Lei Y
  • Sens Actuators Rep 2020[Nov]; 2 (1): 100017 PMID35047828show ga
  • The coronavirus disease 2019 (COVID-19) pandemic has infected millions of people around the globe. The outbreak caused by the novel coronavirus (SARS-CoV-2) poses a great health risk to the public. Therefore, rapid and accurate diagnosis of the virus plays a crucial role in treatment of the disease and saving lives. The current standard method for coronavirus detection is the reverse transcription polymerase chain reaction (RT-PCR) method. However, laboratory-based RT-PCR test for SARS-COV-2 requires complex facilities and elaborate training of operators, thus suffering from limit testing capacity and delayed results. Consequently, isothermal PCR such as loop-mediated isothermal amplification (LAMP) has been emerging as a great alternative to the RT-PCR method. LAMP possesses some fundamental advantages such as amplification at a constant temperature, exclusion of a thermal cycler, a faster test result, and potentially a larger diagnostic capacity, while maintaining similar sensitivity and specificity, thus making it more suitable than the RT-PCR for monitoring a pandemic. Starting with a brief introduction of the working principle of LAMP method, this review summarizes recent progress in LAMP-enabled SARS-CoV-2 viral RNA detection. Lastly, future research directions are discussed. This critical review will motivate biosensor community in furthering the present research, which may pave the road for rapid and large-scale screening of SARS-CoV-2.
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